The stereochemical course of group II intron self-splicing

Science
R A PadgettP S Perlman

Abstract

The stereochemical specificities and reaction courses for both self-splicing steps of a group II intron have been determined by phosphorothioate substitution at the 5' and 3' splice site phosphodiester bonds. Both steps of the splicing reaction proceeded with a phosphorothioate in the Sp configuration but were blocked by the Rp diastereomer. Both steps also proceeded with inversion of stereochemical configuration around phosphorus, consistent with a concerted transesterification reaction. These results are identical to those found for nuclear precursor mRNA (pre-mRNA) splicing and provide support for the hypothesis that group II introns and nuclear pre-mRNA introns share a common evolutionary history.

References

Sep 1, 1990·Trends in Biochemical Sciences·A Jacquier
Jan 1, 1985·Annual Review of Biochemistry·F Eckstein
Sep 1, 1985·Cell·P A Sharp
May 12, 1989·Science·J RajagopalJ W Szostak
Nov 25, 1993·Nucleic Acids Research·K L Maschhoff, R A Padgett
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T A Steitz, J A Steitz
Jan 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Yarus

❮ Previous
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Citations

Jan 1, 1996·Biochimie·A Jacquier
Nov 30, 2002·Biochimie·F Eckstein
Jun 7, 2002·Molecular Cell·Girish C Shukla, Richard A Padgett
Nov 16, 2004·Nature Structural & Molecular Biology·Dipali G SashitalSamuel E Butcher
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·C L PeeblesJ S Franzen
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·J M WarneckeR K Hartmann
May 9, 2001·Nucleic Acids Research·Y TakagiK Taira
Mar 2, 2011·Nucleic Acids Research·Kerstin PetersenRalph Bock
May 14, 2010·Cold Spring Harbor Perspectives in Biology·Alan M Lambowitz, Steven Zimmerly
Jul 10, 1999·Genes & Development·E J SontheimerJ A Piccirilli
Mar 31, 2010·Genes & Development·Venkata R ChalamcharlaMarlene Belfort
Jan 1, 1997·Annual Review of Biochemistry·G J Narlikar, D Herschlag
Oct 6, 1998·Annual Review of Biochemistry·S Verma, F Eckstein
Jun 16, 2007·Biological Chemistry·Olga Fedorova, Nora Zingler
Jan 23, 1999·Current Opinion in Genetics & Development·J M Logsdon
Feb 2, 2010·Nature Chemical Biology·Michael RoitzschAnna Marie Pyle
Aug 12, 2005·Cytogenetic and Genome Research·A R Robart, S Zimmerly
Feb 16, 2016·Wiley Interdisciplinary Reviews. RNA·Bonnie A McNeilSteven Zimmerly
Apr 16, 2014·Chemical Reviews·W Luke WardVictoria J DeRose
Jan 26, 2007·The FEBS Journal·Barbara NawrotWojciech J Stec
May 20, 2011·Chemistry : a European Journal·Tuomas Lönnberg
May 16, 2006·Current Opinion in Structural Biology·Mary R Stahley, Scott A Strobel
Oct 30, 2012·Cell·Marco Marcia, Anna Marie Pyle
Jan 28, 2015·Biochimica Et Biophysica Acta·Christian Schmitz-LinneweberOren Ostersetzer-Biran
May 12, 2015·Mobile DNA·Steven Zimmerly, Cameron Semper
Jul 9, 2016·Annual Review of Biophysics·Anna Marie Pyle
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Abhijit A Patel, Joan A Steitz
Dec 10, 2016·Science·Maria CostaFrançois Michel
Nov 3, 2017·Metallomics : Integrated Biometal Science·Wenhu Zhou, Juewen Liu
Mar 15, 2000·Journal of Molecular Biology·R HillerM W Mueller

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