The sterol-binding antibiotic nystatin differentially modulates ligand binding of the bovine hippocampal serotonin1A receptor

Biochemical and Biophysical Research Communications
Thomas J PucadyilA Chattopadhyay

Abstract

We have monitored the ligand binding of the bovine hippocampal 5-HT1A receptor following treatment with the sterol-binding antifungal antibiotic nystatin. Nystatin considerably inhibits the specific binding of the antagonist to 5-HT1A receptors in a concentration-dependent manner. However, the specific agonist binding does not show significant changes. Fluorescence polarization measurements of membrane probes incorporated at different locations in the membrane revealed a substantial decrease in the membrane order in the interior of the bilayer. Experiments with cholesterol-depleted membranes indicate that the action of nystatin is mediated through membrane cholesterol. These results represent the first report on the effect of a cholesterol-perturbing agent on the ligand-binding activity of this important neurotransmitter receptor.

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Citations

Aug 22, 2007·Cellular and Molecular Neurobiology·Shanti Kalipatnapu, Amitabha Chattopadhyay
Dec 5, 2008·Glycoconjugate Journal·Yamuna Devi Paila, Amitabha Chattopadhyay
Dec 24, 2005·The Journal of Investigative Dermatology·Yasushi OgawaAkira Takashima
Jan 27, 2007·Biochemical and Biophysical Research Communications·Amitabha Chattopadhyay, Yamuna Devi Paila
Sep 11, 2012·Journal of Neurochemistry·Kymry T JonesMaarten E A Reith
Sep 17, 2004·Biochemical and Biophysical Research Communications·Shanti KalipatnapuAmitabha Chattopadhyay
Apr 18, 2006·Progress in Lipid Research·Thomas J Pucadyil, Amitabha Chattopadhyay
Dec 30, 2008·Biochimica Et Biophysica Acta·Yamuna Devi PailaAmitabha Chattopadhyay
Jul 27, 2005·Biochimica Et Biophysica Acta·Soumi Mukherjee, Amitabha Chattopadhyay
Jul 19, 2016·Biochimica Et Biophysica Acta·María Virginia BorroniFrancisco J Barrantes
Oct 23, 2004·FEBS Letters·Shanti Kalipatnapu, Amitabha Chattopadhyay
Jan 6, 2017·Journal of breast cancer·Anil BadanaRama Rao Malla
Nov 26, 2014·Nuclear Receptor Signaling·Carly S FilgueiraPaul Webb
Nov 23, 2006·Cellular and Molecular Neurobiology·Yamuna Devi Paila, Amitabha Chattopadhyay
Aug 2, 2021·Chemistry and Physics of Lipids·Parijat Sarkar, Amitabha Chattopadhyay
Nov 5, 2014·ACS Chemical Neuroscience·Hirak Chakraborty, Amitabha Chattopadhyay
Aug 27, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Francis QuiltyAideen Long

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