The stimulatory effect of insulin-like growth factor-1 on the proliferation, differentiation, and mineralisation of osteoblastic cells from Holstein cattle

The Veterinary Journal
Su-Hua LiGan-Zhen Deng

Abstract

This study investigated the effect of exogenous insulin-like growth factor (IGF)-1 on the proliferation and differentiation of osteoblastic cells from Chinese Holstein cattle and the resultant bone nodule formation and mineralisation in vitro. The osteoblastic cells were isolated and cultured, then identified using Giemsa and alkaline phosphatase (ALP) staining methods. The effect of different concentrations of IGF-1 on cell growth was assessed by MTT assay. The ALP activity and osteocalcin (OC) concentration in the osteoblastic cells were measured by a colorimetric assay and a radioimmmunoassay, respectively. Calcium nodules were observed using alizarin red S stain, while the content of matrix calcium was determined by atomic absorption spectrophotometry. Cell proliferation in the cultures was stimulated by IGF-1 at concentrations ranging from 1 to 200ng/mL, with the maximum effect observed at 100ng/mL. This effect was observed from day 1 and peaked at day 5, but decreased at day 7. At concentrations of 10ng/mL and 100ng/mL, IGF-1 significantly induced ALP activity, OC level, matrix calcium content, and nodule formation of the osteoblastic cells by 20-180% (P<0.05 or P<0.01), compared to controls. The results suggested that IG...Continue Reading

References

Jul 1, 1990·Acta Endocrinologica·E M Rutanen, F Pekonen
Mar 1, 1986·Calcified Tissue International·C G BellowsM E Antosz
Dec 1, 1988·Calcified Tissue International·E CanalisM Centrella
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S M SeyedinK A Piez
Jul 1, 1994·Journal of Cellular Biochemistry·A M DelanyE Canalis
Jun 12, 2003·The International Journal of Biochemistry & Cell Biology·E J Mackie
May 24, 2006·Biochemical and Biophysical Research Communications·Jörg FiedlerRolf E Brenner
Oct 13, 2006·Journal of Ethnopharmacology·Seok-Jong SuhCheorl-Ho Kim

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Citations

Nov 23, 2013·Calcified Tissue International·Petra JufferAstrid D Bakker
Sep 18, 2009·Cellular and Molecular Life Sciences : CMLS·Vincent LaizéM Leonor Cancela
Mar 31, 2011·General and Comparative Endocrinology·Encarnación CapillaJoaquim Gutiérrez
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xue-Dong LiShi-Xin Du
Dec 25, 2010·Journal of Bone and Mineral Metabolism·Daniel M TiagoVincent Laizé
Nov 25, 2011·American Journal of Physiology. Endocrinology and Metabolism·Petra JufferJenneke Klein-Nulend

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