PMID: 2483881Dec 1, 1989Paper

The stimuli releasing histamine from murine bone marrow-derived mast cells. 1. The presence of P2-purinoceptors.

Arerugī = [Allergy]
H NakamuraY Ikura

Abstract

We have examined the histamine-releasing effects of 4 different stimuli on murine bone marrow-derived mast cells (BMMC). We obtained 10(9) BMMC from one femur of a CBA/J mouse when it was cultured in the presence of conditioned medium from WEHI-3 cells for 4 weeks. When these cells were sensitized with monoclonal anti-DNP IgE antibodies, DNP35HSA antigen, ionomycin, thrombin and ATP induced 70%, 70%, 40% and 60% histamine release from BMMC, respectively. Thrombin induced rapid degranulation, whereas the kinetics of histamine release by other stimuli reached a plateau after 5 min. ADP, AMP and GTP as well as ATP but not adenosine caused histamine release from BMMC, suggesting the presence of P2-purinoceptors on these cells.

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