PMID: 6168871Aug 1, 1981Paper

The stimulus-induced release of unmetabolized 5-hydroxytryptamine from superfused rat brain synaptosomes

Journal of Neuroscience Methods
K J CollardR M Taylor

Abstract

A number of existing techniques have been modified to produce an improved technique for examining the depolarization-induced release of unmetabolized 5-hydroxytryptamine (5-HT) from drug-free superfused synaptosomes. Synaptosomes which have been pre-loaded with [3H]5-HT are set up in perfusion chambers and continuously perfused with medium. Serial samples of effluent perfusate are collected on ice and the released 5-HT and 5-hydroxy-indole acetic acid (5-HIAA) are separated by ion exchange chromatography. The technique provides reproducible Ca2+-dependent responses to K+ depolarization in the absence of monoamineoxidase inhibition and free from the interference of re-uptake. The advantages of the use of the system in examining transmitter release and its modulation is discussed.

References

Aug 15, 1979·European Journal of Pharmacology·F Cerrito, M Raiteri
Jan 6, 1978·Brain Research·J C Reubi, P C Emson
Mar 1, 1974·European Journal of Pharmacology·M RaiteriG Levi
Jan 1, 1972·Annual Review of Biochemistry·Z W Hall
Nov 1, 1974·European Journal of Pharmacology·K J Collard, M H Roberts
Jul 1, 1972·Journal of Neurochemistry·J S De Belleroche, H F Bradford
Mar 1, 1972·Journal of Neurochemistry·J S De Belleroche, H F Bradford

❮ Previous
Next ❯

Citations

Aug 1, 1985·Journal of Neuroscience Methods·D Minnema, I A Michaelson
Jan 15, 1993·Brain Research. Developmental Brain Research·K J CollardY Liu
Sep 1, 1990·Brain Research Bulletin·E C PetrieJ G Csernansky
May 12, 2000·British Journal of Pharmacology·S SbrennaC Bianchi
Dec 1, 1990·British Journal of Pharmacology·L S Wilkinson, K J Collard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.