The straight and curved conformation of FtsZ protofilaments-evidence for rapid exchange of GTP into the curved protofilament

Cell Structure and Function
C Lu, Harold P Erickson

Abstract

Bacterial cell division protein FtsZ assembles into protofilaments, which can adopt a straight or curved conformation, similar to its eukaryotic homolog, tubulin. The straight protofilaments can assemble into sheets with a lattice similar to the microtubule wall. The curved protofilaments can form rings when adsorbed to a lipid monolayer, but in solution they form helices. 4 helices assemble together to make a tube, the characteristic polymer of the curved protofilament. GTP favors the straight conformation, while GDP favors the curved. We show here that addition of EDTA and GTP to tubes causes a rapid transformation to straight protofilament sheets. Apparently when the magnesium is chelated the GDP in the curved protofilaments dissociates rapidly and is replaced with GTP, and this GTP induces the transition to straight protofilaments.

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Citations

Dec 30, 1999·Journal of Bacteriology·C LuH P Erickson
Jul 24, 2007·The Journal of Biological Chemistry·Yaodong ChenHarold P Erickson
Feb 21, 2009·Bulletin of Mathematical Biology·Donald A DrewOswaldo Sanchez
Jun 8, 2006·Annual Review of Biochemistry·Katharine A Michie, Jan Löwe
Nov 15, 2000·Biological Chemistry·J Löwe, L A Amos
Oct 17, 2012·Trends in Cell Biology·Ariadna MartosPetra Schwille
Oct 3, 2012·Cytoskeleton·Mohan K BalasubramanianKian-Hong Ng
Jun 3, 2011·Molecular Microbiology·Masaki Osawa, Harold P Erickson
Nov 19, 2011·FEMS Microbiology Reviews·Michael Ingerson-Mahar, Zemer Gitai
Mar 7, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Claire E DowHugo A van den Berg
Dec 3, 2014·Nihon saikingaku zasshi. Japanese journal of bacteriology·Daisuke Shiomi
Jun 18, 2017·Scientific Reports·Yaodong ChenHarold P Erickson

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