PMID: 19937575Nov 26, 2009Paper

The strain differentiation of Candida albicans by morphotyping

Klinická mikrobiologie a infekc̆ní lékar̆ství
Petr Hamal, Dagmar Koukalová

Abstract

Morphotyping is a phenotypic method for strain differentiation of yeast cultures based on the comparison of appearance of their surfaces and fringes. For its simplicity and cost-effectiveness, it is recommended as an alternative tool to genotyping of Candida albicans. Our study aimed at verifying its usefulness for typing a group of C. albicans clinical isolates with emphasis on discrimination power. Prevalence of specific morphotypes in body locations with more tested isolates and according to age groups and gender of patients was also evaluated. The tested group comprised 97 C. albicans isolates from 93 patients. With a cotton swab, 24-hour cultures were streaked onto malt extract agar. The surface and fringe morphology was assessed after 10-day incubation at 30 degrees C. A total of 30 morphotypes were detected. The most frequent one had a code profile 7240 (n = 13, 13.4 %), followed by 5240 (n = 11) and 7340 (n = 9). The discrimination power calculated by Simpson's index of diversity reached 0.95. Results of reproducibility testing were identical in 54.6 % of isolates, another 36.1 % of isolates differed in one character. Some isolates (n = 35) were biotyped by our modification of Odds method. Using morphological criteria, ...Continue Reading

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