The strain-specific cis-acting element of beet curly top geminivirus DNA replication maps to the directly repeated motif of the ori

Virology
I R Choi, D C Stenger

Abstract

Strains of beet curly top geminivirus (BCTV) possess distinct cis- and trans-acting replication specificity elements which are not separately interchangeable among strains. Analysis of the replication competency of chimeric BCTV genomes, in which portions of the origin of DNA replication (ori) were derived from heterologous BCTV strains, have permitted identification of an essential cis-acting element governing strain-specific replication in a subgroup II geminivirus. Our studies indicate that the cis-acting element responsible for strain-specific replication properties resides within the directly repeated motif of the BCTV ori. Transient replication assays conducted in leaf disks and complementation experiments conducted in whole plants indicated that the trans-acting replication specificity element, residing within the amino-terminal region of the C1 Rep protein, may recognize and replicate a chimeric BCTV genome containing a heterologous ori so long as all or portions of the core element of the directly repeated motif are derived from the same strain as the Rep protein. As Rep protein binding to the core element of the directly repeated motif has been demonstrated by others to be essential for replication of subgroup III gem...Continue Reading

Citations

Oct 24, 2008·Phytopathology·C A StrausbaughI A Eujayl
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Oct 14, 2011·Viruses·Darren P MartinArvind Varsani
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