The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device

Biosensors & Bioelectronics
Ji QiLingxin Chen

Abstract

In this work, we provided a novel strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers (MIPs) on movable valve microfluidic paper-based electrochemical device (Bio-MIP-ePADs) for clinical detection of biomarkers. The newly movable valves on the device enable continuous and convenient delivery of fluid, which guarantee the performance for fabricating MIPs structure during long time electropolymerization. Moreover, this strategy can directly detect antigens by taking advantage of molecular imprinting on paper-based device, which greatly decreases the cost during clinical testing, reduces the tedious washing procedure and does not need to consider the preservation of the antibody in enzyme linked immunosorbent assay (ELISA). This feature makes the chip suitable for the on-site family treatment or commercial products. To further validate the applicability of this proposed method for clinical diagnostic testing, carcinoembryonic antigen (CEA) was applied as prototyping model target for the clinical analysis. The proposed Bio-MIP-ePADs were cheap, easy to prepare, disposable and provided reliable analysis by comparing with ELISA. We hope the application of this technology will open up a n...Continue Reading

Citations

Feb 15, 2020·Sensors·Manuel Gutiérrez-CapitánCésar Fernández-Sánchez
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·M C Díaz-LiñánA I López-Lorente
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Shokoufeh HassaniMohammad Abdollahi
Oct 16, 2020·Scientific Reports·Mohammad Mahdi BordbarHasan Bagheri
Feb 18, 2021·Talanta·Jiaqi BuShian Zhong
Feb 27, 2021·ACS Sensors·Sujay K BiswasSuman Chakraborty
Mar 2, 2021·Journal, Genetic Engineering & Biotechnology·Amruta Patil-JoshiAnjali Apte-Deshpande
Dec 2, 2020·Journal of Hazardous Materials·Shishir VenkateshVellaisamy A L Roy
Apr 17, 2021·Analytical Methods : Advancing Methods and Applications·Toyohiro NaitoKoji Otsuka
Jun 20, 2021·Advanced Materials·Maryam ArabiLingxin Chen
Jan 16, 2021·ACS Applied Materials & Interfaces·Tomás PinheiroElvira Fortunato
Nov 26, 2021·Analytical Methods : Advancing Methods and Applications·Tao ZhangYiyuan Zhang

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