The strength of the protein-material interaction determines cell fate

Acta Biomaterialia
Cristina González-GarcíaManuel Salmerón-Sánchez

Abstract

Extracellular matrix (ECM) proteins are key mediators of cell/material interactions. The surface density and conformation of these proteins adsorbed on the material surface influence cell adhesion and the cellular response. We have previously shown that subtle variations in surface chemistry lead to drastic changes in the conformation of adsorbed fibronectin (FN). On poly(ethyl acrylate) (PEA), FN unfolds and displays domains for cell adhesion and FN-FN interaction, whereas on poly(methyl acrylate) (PMA) - with only one methyl group less - FN remains globular as it is in solution. The effect of the strength of the protein/material interaction in cell response, and its relation to protein density and conformation, has received limited attention so far. In this work, we used FN-functionalized AFM cantilevers to evaluate, via force spectroscopy, the strength of interaction between fibronectin and the underlying polymer which controls FN conformation (PEA and PMA). We found that the strength of FN/PEA interaction is significantly higher than FN/PMA, which limits the mobility of FN layer on PEA, reduces the ability of cells to mechanically reorganize FN and then leads to enhanced proteolysis and degradation of the surrounding matrix...Continue Reading

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Citations

Oct 30, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xize GaoJun Wu
Nov 8, 2020·Journal of Materials Science. Materials in Medicine·Mei-Chen LinYueh-Sheng Chen
Dec 29, 2020·Frontiers in Chemistry·Alexander H Jesmer, Ryan G Wylie
Jan 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ruben DaumKatja Schenke-Layland
Mar 22, 2020·Biomaterials·Jefferson FrigugliettiFatima A Merchant
Mar 12, 2021·Biotechnology and Bioengineering·Mingji WeiYecheng Zhang

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