The Streptomyces coelicolor GlnR regulon: identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes

Molecular Microbiology
Yvonne TiffertJens Reuther

Abstract

Streptomyces coelicolor GlnR is a global regulator that controls genes involved in nitrogen metabolism. By genomic screening 10 new GlnR targets were identified, including enzymes for ammonium assimilation (glnII, gdhA), nitrite reduction (nirB), urea cleavage (ureA) and a number of biochemically uncharacterized proteins (SCO0255, SCO0888, SCO2195, SCO2400, SCO2404, SCO7155). For the GlnR regulon, a GlnR binding site which comprises the sequence gTnAc-n(6)-GaAAc-n(6)-GtnAC-n(6)-GAAAc-n(6) has been found. Reverse transcription analysis of S. coelicolor and the S. coelicolor glnR mutant revealed that GlnR activates or represses the expression of its target genes. Furthermore, glnR expression itself was shown to be nitrogen-dependent. Physiological studies of S. coelicolor and the S. coelicolor glnR mutant with ammonium and nitrate as the sole nitrogen source revealed that GlnR is not only involved in ammonium assimilation but also in ammonium supply. blast analysis demonstrated that GlnR-homologous proteins are present in different actinomycetes containing the glnA gene with the conserved GlnR binding site. By DNA binding studies, it was furthermore demonstrated that S. coelicolor GlnR is able to interact with these glnA upstream...Continue Reading

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Citations

Jan 14, 2011·Applied Microbiology and Biotechnology·Yvonne TiffertYvonne Mast
May 25, 2012·Applied Microbiology and Biotechnology·Juan F MartínEllizabeth M H Wellington
Jul 31, 2012·Applied and Environmental Microbiology·Toju IinoMasao Fukuda
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May 7, 2013·BMC Genomics·Victoria A JenkinsKerstin J Williams
Dec 18, 2013·Fungal Genetics and Biology : FG & B·Ling ChenZhihua Zhou
Jun 17, 2014·Applied Microbiology and Biotechnology·Li-Li YaoBang-Ce Ye
Jan 9, 2013·Journal of Bioscience and Bioengineering·Toju IinoMasao Fukuda
Oct 1, 2011·Proteomics·Andris JankevicsRainer Breitling
Feb 24, 2011·Microbial Biotechnology·Juan F MartínAntonio Rodríguez-García
Jun 4, 2010·Protein Science : a Publication of the Protein Society·Nishant D ShettyJames C Sacchettini
Jun 17, 2015·Molecular Microbiology·Kerstin J WilliamsBrian D Robertson

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