The Streptomyces coelicolor sporulation-specific sigma WhiG form of RNA polymerase transcribes a gene encoding a ProX-like protein that is dispensable for sporulation

Gene
H TanK F Chater

Abstract

In the non-motile mycelial organism Streptomyces coelicolor A3(2), the sporulation gene whiG encodes a protein that closely resembles RNA polymerase sigma factors such as sigma D of Bacillus subtilis, which mainly control motility and chemotaxis genes. Here, we show that the whiG gene product, purified from an Escherichia coli strain carrying an expression construct, could activate E. coli core RNA polymerase in vitro to transcribe a sigma D-dependent motility-related promoter from B. subtilis. Such RNA polymerase holoenzyme preparations could also transcribe from an S. coelicolor promoter, PTH4, previously shown to require an intact whiG gene for in-vivo transcription. The in-vivo dependence on whiG was therefore shown to be direct. Unusually, the initiation of PTH4 transcription in vitro depended on the provision of appropriate dinucleotides. The whiG-dependent PTH4 transcription unit consisted of a single gene, orfTH4. Sequence comparisons suggested that the gene product was a member of a small group of proteins that include the B. subtilis and E. coli ProX proteins. Though none of these proteins shared more than about 30% of extended primary sequence identity, they had similar size and hydropathy profiles, and could be alig...Continue Reading

References

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Citations

Aug 30, 2008·Science in China. Series C, Life Sciences·H YangK F Chater
Aug 30, 2008·Science in China. Series C, Life Sciences·Y TianK F Chater
Oct 11, 2007·Current Microbiology·Xue-Ling ShenYong-Quan Li
Jun 15, 1999·Trends in Pharmacological Sciences·D L Ennist
Dec 4, 2001·Current Opinion in Microbiology·K F Chater
Jul 14, 2000·Biomolecular Engineering·H XiangH Tan
Jan 12, 2013·Bioconjugate Chemistry·Peerada YingyuadAndrew D Miller
Mar 1, 2015·Applied Microbiology and Biotechnology·Shui-Ping LiuYong-Quan Li
Aug 17, 2006·FEMS Microbiology Reviews·Keith F Chater, Govind Chandra
Sep 28, 2010·Microbiological Research·Hum Nath JnawaliJae Kyung Sohng
Dec 24, 2005·The Journal of Antimicrobial Chemotherapy·Estelle AndréJean-Paul Leonetti

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