The stressed bird in the hand: Influence of sampling design on the physiological stress response in a free-living songbird.

Physiology & Behavior
Nikolaus HuberÁdám Z Lendvai

Abstract

Despite the widely used application of standardized capture-handling protocols to collect blood and assess the physiological stress response, the actual sampling design (e.g., timing and the number of blood samples) often differs between studies, and the potential implications for the measured physiological endpoints remain understudied. We, therefore experimentally tested the effects of repeated handling and multiple blood sampling on the stress response in wintering free-living great tits (Parus major). We modified a well-established sampling protocol of avian studies by adding either an additional blood sample or a "sham-manipulation" (i.e., handling associated with the blood sampling procedure without venepuncture), to disentangle the effects of handling stress and blood loss. We combined three different stress metrics along the endocrine-immune interface to investigate the acute short-term stress response: total corticosterone concentrations (Cort), the heterophil/lymphocyte ratio (H:L), and the Leucocyte Coping Capacity (LCC). Our study provided three key results: i) no relationship between Cort levels, LCC and H:L, confirming that these three parameters represent different physiological endpoints within the stress respon...Continue Reading

References

Mar 12, 1987·Journal of Immunological Methods·H Gyllenhammar
Jul 7, 2000·General and Comparative Endocrinology·L M Romero, L Remage-Healey
Jan 25, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·L Michael Romero, J Michael Reed
Mar 17, 2007·Bulletin of Experimental Biology and Medicine·V A FrolovA V Pasechnik
May 15, 2007·Nature·Manuel ThéryFrank Jülicher
Sep 23, 2008·Ecology Letters·David Costantini
Feb 11, 2011·The Journal of Experimental Biology·Adám Z LendvaiOlivier Chastel
Feb 24, 2011·Oecologia·Michael J SheriffRudy Boonstra
Mar 15, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Christopher P JohnstoneAlan Lill
Jan 10, 2013·General and Comparative Endocrinology·John F Cockrem
Feb 26, 2013·Physiological and Biochemical Zoology : PBZ·Jesse J PakkalaAmy E M Newman
Jan 28, 2015·British Poultry Science·T L LentferE Von Borell
Jun 15, 2016·Developmental and Comparative Immunology·B M Lidy Verburg-van KemenadeMagdalena Chadzinska
Dec 20, 2018·International Journal of Molecular Sciences·Simona RonchettiCarlo Riccardi
Apr 30, 2019·Frontiers in Veterinary Science·Nikolaus HuberChris Walzer
Jul 23, 2019·General and Comparative Endocrinology·Brenna M G GormallyL Michael Romero
Feb 27, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Xavier BonnetMargareta Lakušić
Feb 27, 2020·Integrative and Comparative Biology·Sarah Guindre-Parker
Apr 6, 2021·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·L Michael Romero, Ursula K Beattie

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