The Stringent Response-Regulated sRNA Transcriptome of Borrelia burgdorferi

Frontiers in Cellular and Infection Microbiology
Dan DrecktrahD S Samuels

Abstract

The Lyme disease spirochete Borrelia (Borreliella) burgdorferi must tolerate nutrient stress to persist in the tick phase of its enzootic life cycle. We previously found that the stringent response mediated by RelBbu globally regulates gene expression to facilitate persistence in the tick vector. Here, we show that RelBbu regulates the expression of a swath of small RNAs (sRNA), affecting 36% of previously identified sRNAs in B. burgdorferi. This is the first sRNA regulatory mechanism identified in any spirochete. Threefold more sRNAs were RelBbu-upregulated than downregulated during nutrient stress and included antisense, intergenic and 5' untranslated region sRNAs. RelBbu-regulated sRNAs associated with genes known to be important for host infection (bosR and dhhp) as well as persistence in the tick (glpF and hk1) were identified, suggesting potential mechanisms for post-transcriptional regulation of gene expression.

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Citations

Nov 28, 2018·Journal of Bacteriology·William K BoyleTravis J Bourret
Nov 20, 2019·Molecular Microbiology·Dan DrecktrahD Scott Samuels
Apr 1, 2020·Molecular Microbiology·Charlotte MasonZhiming Ouyang
Jul 3, 2020·Frontiers in Molecular Biosciences·Juan José González Plaza
Oct 6, 2020·Frontiers in Microbiology·Diego A Vargas-Blanco, Scarlet S Shell

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Datasets Mentioned

BETA
SR0175
SRX971832
SRX971835

Methods Mentioned

BETA
RNA-seq

Software Mentioned

edgeR
NextGenMap
CODOC
FeatureCounts

Related Concepts