The structural and functional contributions of β-glucosidase-producing microbial communities to cellulose degradation in composting

Biotechnology for Biofuels
Xiangyun ZangHong-Tao Li

Abstract

Compost habitats sustain a vast ensemble of microbes that engender the degradation of cellulose, which is an important part of global carbon cycle. β-Glucosidase is the rate-limiting enzyme of degradation of cellulose. Thus, analysis of regulation of β-glucosidase gene expression in composting is beneficial to a better understanding of cellulose degradation mechanism. Genetic diversity and expression of β-glucosidase-producing microbial communities, and relationships of cellulose degradation, metabolic products and the relative enzyme activity during natural composting and inoculated composting were evaluated. Compared with natural composting, adding inoculation agent effectively improved the degradation of cellulose, and maintained high level of the carboxymethyl cellulose (CMCase) and β-glucosidase activities in thermophilic phase. Gene expression analysis showed that glycoside hydrolase family 1 (GH1) family of β-glucosidase genes contributed more to β-glucosidase activity in the later thermophilic phase in inoculated compost. In the cooling phase of natural compost, glycoside hydrolase family 3 (GH3) family of β-glucosidase genes contributed more to β-glucosidase activity. Intracellular β-glucosidase activity played a cruci...Continue Reading

References

Oct 15, 1992·European Journal of Biochemistry·T WatanabeM Kuwahara
May 1, 1996·Applied and Environmental Microbiology·T BeffaM Aragno
Aug 30, 2002·Applied and Environmental Microbiology·Markku SaloheimoMerja Penttilä
Dec 19, 2002·Critical Reviews in Biotechnology·Yukti BhatiaV S Bisaria
Jan 1, 1992·Applied and Environmental Microbiology·T KurasawaF Tomita
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Sep 17, 2009·Applied Microbiology and Biotechnology·Kristian B R M KroghLisbeth Olsson
Aug 23, 2011·Journal of Bioscience and Bioengineering·Juanjuan LiuYazhong Xiao
Oct 17, 2012·Bioresource Technology·Reeta Rani SinghaniaAshok Pandey
May 18, 2013·Biotechnology for Biofuels·Etienne JourdierFadhel Ben Chaabane
Jun 5, 2013·Journal of Industrial Microbiology & Biotechnology·Kuni Parambil RajasreeRajeev Kumar Sukumaran
Jun 11, 2014·Acta Crystallographica. Section D, Biological Crystallography·Priscila Oliveira de GiuseppeMario Tyago Murakami
Feb 5, 2015·Biotechnology for Biofuels·Christopher W SimmonsJean S VanderGheynst

❮ Previous
Next ❯

Citations

Mar 22, 2019·Journal of Applied Microbiology·J D Fernández-BayoJ S VanderGheynst
Aug 23, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Chein-Chi Chang, Rundong Li
Sep 11, 2020·Environmental Science and Pollution Research International·Matteo GarauGiovanni Garau
Oct 23, 2020·Biotechnology for Biofuels·Xinyue ZhangHongtao Li
Sep 25, 2021·Applied Biochemistry and Biotechnology·Francinaldo Leite da SilvaEveraldo Silvino Dos Santos

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
transglycosylation

Software Mentioned

Primer
BLAST
Graphpad Prism

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.