PMID: 9428519Jan 15, 1998Paper

The structural basis for 14-3-3:phosphopeptide binding specificity

Cell
Michael B YaffeL C Cantley

Abstract

The 14-3-3 family of proteins mediates signal transduction by binding to phosphoserine-containing proteins. Using phosphoserine-oriented peptide libraries to probe all mammalian and yeast 14-3-3s, we identified two different binding motifs, RSXpSXP and RXY/FXpSXP, present in nearly all known 14-3-3 binding proteins. The crystal structure of 14-3-3zeta complexed with the phosphoserine motif in polyoma middle-T was determined to 2.6 A resolution. The bound peptide is in an extended conformation, with a tight turn created by the pS +2 Pro in a cis conformation. Sites of peptide-protein interaction in the complex rationalize the peptide library results. Finally, we show that the 14-3-3 dimer binds tightly to single molecules containing tandem repeats of phosphoserine motifs, implicating bidentate association as a signaling mechanism with molecules such as Raf, BAD, and Cbl.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jul 5, 1990·Journal of Molecular Biology·D E StewartJ E Wampler
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Bonnefoy-BérardA Altman
Mar 12, 1993·Cell·Z SongyangR J Lechleider
Jan 27, 1995·Journal of Molecular Biology·D H JonesA Aitken
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·K S CampbellD C Pallas
Jan 10, 1997·The Journal of Biological Chemistry·K NishikawaL C Cantley
Mar 28, 1997·The Journal of Biological Chemistry·H WakuiJ Zilliacus
Apr 1, 1997·Journal of Molecular Graphics & Modelling·R M Esnouf
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

❮ Previous
Next ❯

Citations

Oct 13, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J van HemertG P van Heusden
Oct 3, 2002·Proteins·Amy B TruongHaian Fu
May 10, 2001·International Journal of Hematology·Y Maru
Jul 12, 2008·Cancer Immunology, Immunotherapy : CII·Ryuji KobayashiRalph S Freedman
Dec 2, 2010·Planta·Xuyan Li, Sangeeta Dhaubhadel
Jun 26, 2012·Cancer Metastasis Reviews·Rémi LonguespéeMichel Salzet
Nov 5, 2005·Plant Molecular Biology·Marco LallePatrizia Aducci
Apr 6, 2004·Comptes rendus biologies·Alphonse GarciaAngelita Rebollo
Mar 17, 2004·Journal of Molecular Biology·Tsaffrir ZorPeter E Wright
Mar 17, 2004·Experimental Cell Research·Romesh R SubramanianHaian Fu
Mar 21, 2003·Biochemical and Biophysical Research Communications·Jernej SribarIgor Krizaj
Sep 21, 2000·Mechanisms of Ageing and Development·W E MüllerH C Schröder
Jun 4, 1999·Progress in Biophysics and Molecular Biology·Z Songyang
Jul 12, 2003·Brain Research. Developmental Brain Research·Jihui ZhangA Joseph D'Ercole
Sep 7, 1999·Molecular and Biochemical Parasitology·B Al-KhederyM R Galinski
Jun 19, 2001·Biochimica Et Biophysica Acta·V A Smits, R H Medema
Jun 19, 2001·Biochimica Et Biophysica Acta·H YangH Fu
Sep 21, 2000·Plant Science : an International Journal of Experimental Plant Biology·T Kuromori, M Yamamoto
Oct 6, 2001·Peptides·M Perego, J A Brannigan
Sep 27, 2000·Biochimica Et Biophysica Acta·S SawasdikosolS J Burakoff
Mar 15, 2001·Current Opinion in Cell Biology·M B Yaffe, A E Elia
Apr 21, 1999·Current Opinion in Cell Biology·T P Garrington, G L Johnson
Sep 8, 1998·Current Opinion in Genetics & Development·C Rommel, E Hafen
Jun 14, 2000·Current Opinion in Neurobiology·A T Brunger
Apr 12, 2001·Current Opinion in Structural Biology·A T Brunger
Nov 24, 2001·Trends in Cell Biology·T PawsonP Nash
Jan 28, 2003·Current Opinion in Chemical Biology·Benjamin E Turk, Lewis C Cantley
Oct 6, 2000·Current Opinion in Plant Biology·M R Roberts
Feb 20, 2010·Biochemistry·Silvia Vega-Rubin-de-CelisXuewu Zhang
Feb 20, 2007·Journal of the American Chemical Society·Vyas SharmaDavid S Lawrence
Apr 13, 2010·Journal of the American Chemical Society·Richard S AgnesDavid S Lawrence
Apr 5, 2012·Journal of Proteome Research·Sofia WaldemarsonPeter James
Mar 29, 2012·Journal of Proteome Research·Marco LalleEdoardo Pozio

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.