The structural basis of 5' triphosphate double-stranded RNA recognition by RIG-I C-terminal domain.

Structure
Cheng LuPingwei Li

Abstract

RIG-I is a cytosolic sensor of viral RNA that plays crucial roles in the induction of type I interferons. The C-terminal domain (CTD) of RIG-I is responsible for the recognition of viral RNA with 5' triphosphate (ppp). However, the mechanism of viral RNA recognition by RIG-I is still not fully understood. Here, we show that RIG-I CTD binds 5' ppp dsRNA or ssRNA, as well as blunt-ended dsRNA, and exhibits the highest affinity for 5' ppp dsRNA. Crystal structures of RIG-I CTD bound to 5' ppp dsRNA with GC- and AU-rich sequences revealed that RIG-I recognizes the termini of the dsRNA and interacts with the 5' ppp through extensive electrostatic interactions. Mutagenesis and RNA-binding studies demonstrated that similar binding surfaces are involved in the recognition of different forms of RNA. Mutations of key residues at the RNA-binding surface affected RIG-I signaling in cells.

References

Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Nov 11, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
May 2, 2006·Nature Biotechnology·Joao Trindade MarquesBryan R G Williams
Sep 19, 2006·Immunity·Daniel B Stetson, Ruslan Medzhitov
Oct 14, 2006·Science·Andreas PichlmairCaetano Reis e Sousa
Oct 14, 2006·Science·Veit HornungGunther Hartmann
Aug 2, 2007·Immunology and Cell Biology·Alex J V Thompson, Stephen A Locarnini
Sep 26, 2007·Immunity·Andreas Pichlmair, Caetano Reis e Sousa
Feb 5, 2008·Molecular Cell·Sheng CuiKarl-Peter Hopfner
Apr 16, 2008·The Journal of Biological Chemistry·Ayaluru MuraliC Cheng Kao
Nov 21, 2008·The Journal of Biological Chemistry·C T Ranjith-KumarCheng C Kao
Jan 6, 2009·Immunological Reviews·Mitsutoshi Yoneyama, Takashi Fujita
Jan 6, 2009·Immunological Reviews·Martin SchleeGunther Hartmann
Jan 6, 2009·Immunological Reviews·Osamu Takeuchi, Shizuo Akira
Jan 6, 2009·Immunological Reviews·Noah W Palm, Ruslan Medzhitov
Feb 12, 2009·Nucleic Acids Research·Diana A PippigKarl-Peter Hopfner
Mar 13, 2009·The Journal of Biological Chemistry·Xiaojun LiPingwei Li
Jun 18, 2009·Archives of Biochemistry and Biophysics·Xiaojun LiPingwei Li
Jul 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andreas SchmidtSimon Rothenfusser
Jul 17, 2009·Immunity·Takashi Fujita
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Takashi SatohOsamu Takeuchi
Feb 11, 2010·Cell·Jan RehwinkelCaetano Reis e Sousa

❮ Previous
Next ❯

Citations

Oct 11, 2013·Molecular Biology and Evolution·Krishanu MukherjeeBryan Kolaczkowski
Dec 17, 2010·The Journal of Biological Chemistry·Jean-Baptiste MarqDominique Garcin
Sep 3, 2011·Microbiology and Molecular Biology Reviews : MMBR·Denis Gerlier, Douglas S Lyles
Oct 14, 2011·Journal of Virology·Chennareddy V Subba-ReddyC Cheng Kao
Oct 12, 2012·The Journal of Biological Chemistry·Adriana VelaAnna Marie Pyle
Nov 3, 2012·RNA·Daniel KolakofskyStephen Cusack
Jan 18, 2013·Nucleic Acids Research·Simone A BeckhamMatthew C J Wilce
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xunyang GuoRui Lu
Jan 19, 2011·Cell Research·Matthew S Hayden, Sankar Ghosh
Sep 11, 2012·Cellular and Molecular Life Sciences : CMLS·Alys Peisley, Sun Hur
Jul 31, 2013·Immunobiology·Martin Schlee
Dec 3, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Koji OnomotoTakashi Fujita
Nov 10, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Li-Wen LiuYasufumi Kaneda
Jan 7, 2016·Proceedings of the National Academy of Sciences of the United States of America·Swapnil C DevarkarJoseph Marcotrigiano
Nov 28, 2015·Nucleic Acids Research·Anand RamanathanSmita S Patel
Jan 10, 2016·Journal of Biochemistry·Mitsutoshi YoneyamaKoji Onomoto
Feb 12, 2013·Trends in Biochemical Sciences·Tom P Monie
May 9, 2012·Current Opinion in Structural Biology·Daisy W Leung, Gaya K Amarasinghe
Apr 10, 2012·Current Opinion in Virology·Kathryn M HastieErica Ollmann Saphire
Mar 24, 2012·Current Opinion in Virology·Melissa Swiecki, Marco Colonna
Oct 27, 2011·Virus Research·Adolfo García-Sastre
Apr 13, 2011·European Journal of Cell Biology·Katharina Eisenächer, Anne Krug
Apr 19, 2011·Trends in Biochemical Sciences·J Bradford BowzardSuryaprakash Sambhara
Apr 19, 2011·European Journal of Cell Biology·Andreas SchmidtKarl-Peter Hopfner
Feb 14, 2015·Virus Research·Marian J KillipRichard E Randall
Sep 3, 2011·Immunological Reviews·Hiroki KatoTakashi Fujita
Jul 13, 2013·EMBO Reports·Jenish R PatelAdolfo García-Sastre
Jul 31, 2013·EMBO Reports·Andrew KohlwayAnna Marie Pyle
Sep 28, 2015·Trends in Biochemical Sciences·Sadeem Ahmad, Sun Hur
Jul 16, 2014·Cytokine & Growth Factor Reviews·Jessica J ChiangMichaela U Gack
May 28, 2013·Current Opinion in Microbiology·Jan Rehwinkel, Caetano Reis e Sousa
Jun 1, 2014·Current Opinion in Structural Biology·David C Rawling, Anna Marie Pyle
Oct 9, 2013·Vaccine·Xiomara Mercado-LópezCarolina B López

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.