The structural basis of actin interaction with multiple WH2/beta-thymosin motif-containing proteins

Structure
Adeleke Halilu AgudaRobert C Robinson

Abstract

Participation of actin in cellular processes relies on the dynamics of filament assembly. Filament elongation is fed by monomeric actin in complex with either profilin or a Wiscott-Aldrich syndrome protein (WASP) homology domain 2 (WH2)/beta-thymosin (betaT) domain. WH2/betaT motif repetition (typified by ciboulot) or combination with nonrelated domains (as found in N-WASP) results in proteins that yield their actin to filament elongation. Here, we report the crystal structures of actin bound hybrid proteins, constructed between gelsolin and WH2/betaT domains from ciboulot or N-WASP. We observe the C-terminal half of ciboulot domain 2 bound to actin. In solution, we show that cibolout domains 2 and 3 bind to both G- and F-actin, and that whole ciboulot forms a complex with two actin monomers. In contrast, the analogous portion of N-WASP WH2 domain 2 is detached from actin, indicating that the C-terminal halves of the betaT and WH2 motifs are not functionally analogous.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Sep 6, 1990·Nature·W KabschK C Holmes
Sep 6, 1990·Nature·K C HolmesW Kabsch
Dec 1, 1993·European Journal of Biochemistry·M CzischT A Holak
Apr 1, 1993·Archives of Biochemistry and Biophysics·S W ReidL D Burtnick
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·R M Esnouf
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·T D PollardR D Mullins
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·H YamaguchiT Takenawa
Jul 28, 2001·Science·L R OtterbeinR Dominguez
Feb 22, 2002·The Journal of Biological Chemistry·Maud HertzogMarie-France Carlier
Mar 26, 2002·FEBS Letters·Eija PaunolaPekka Lappalainen
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Matthew D Welch, R Dyche Mullins
Jul 23, 2003·Nature Structural Biology·Sanjay C PanchalMichael K Rosen
Jun 25, 2004·The EMBO Journal·Leslie D BurtnickRobert C Robinson
Jul 13, 2004·Trends in Cell Biology·Ville O PaavilainenPekka Lappalainen
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Aug 26, 2004·FEBS Letters·John Edwards
Aug 27, 2004·The EMBO Journal·Edward IrobiRobert C Robinson
Jan 28, 2005·Nature·Margot E QuinlanR Dyche Mullins
Nov 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·David ChereauRoberto Dominguez

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Citations

Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Bo XueRobert C Robinson
Nov 26, 2013·European Journal of Cell Biology·Bo Xue, Robert C Robinson
Sep 23, 2014·Journal of Molecular Biology·Amy S RassonMargot E Quinlan
May 1, 2009·Cell Motility and the Cytoskeleton·Hans Georg Mannherz, Ewald Hannappel
Oct 20, 2007·Annals of the New York Academy of Sciences·Marie-France CarlierEric Guittet
May 1, 2007·Annals of the New York Academy of Sciences·Bo XueRobert Charles Robinson
Jun 12, 2010·Annals of the New York Academy of Sciences·Clotilde HussonMarie-France Carlier
Jun 4, 2013·Cell Reports·Xiaorui ChenJianpeng Ma
Jan 8, 2013·Current Biology : CB·Agnieszka N UrbanekKathryn R Ayscough
Nov 29, 2007·Molecular Cell·Montserrat BoschMarie-France Carlier
Feb 24, 2009·Journal of Molecular Biology·Stien DhaeseMarleen Van Troys
May 30, 2006·Biophysical Journal·Richard B Dickinson, Daniel L Purich
May 29, 2007·Biophysical Journal·Xiange ZhengDavid Sept
Aug 1, 2018·Expert Opinion on Biological Therapy·Walter MandalitiMaurizio Paci
Apr 13, 2019·Journal of Cellular Biochemistry·Eric W Miller, Scott D Blystone
Jun 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anna M DuckaTomasz Sitar
Oct 9, 2019·International Journal of Molecular Sciences·Jie JingXiuyue Zhang
Apr 20, 2019·International Journal of Biological Macromolecules·Priyanka DuttaSankar Maiti

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