PMID: 6408226Jul 1, 1983Paper

The structural conservation of S100 protein during evolution: analysis by reactivity with a monoclonal antibody

Journal of Neurochemistry
A MarksR Baumal

Abstract

A hybridoma cell line producing a monoclonal antibody (A4) against bovine S100 protein has been produced by fusing mouse myeloma P3X63/Ag8 cells with spleen cells from a BALB/c mouse immunized with bovine S100 protein. A4 is of the IgG2b subclass and was purified by affinity chromatography on a protein A-Sepharose column. Brain extracts from several mammalian and one avian species reacted both with polyclonal rabbit anti-S100 protein antiserum and with A4 in a radioimmunoassay. Brain extract from dog was a notable exception. It reacted with the rabbit antiserum but not with A4. Therefore A4 reacts with a common epitope that is present on S100 proteins from different vertebrate species but is absent on dog S100 protein.

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Citations

Oct 24, 2002·Brain Research. Molecular Brain Research·Richard H DyckG Campbell Teskey
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L J Van EldikR A Jensen
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D BaileyA Marks
Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Marks, R Allore
Jun 1, 1986·Cell Calcium·R Donato

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