PMID: 6408226Jul 1, 1983Paper
The structural conservation of S100 protein during evolution: analysis by reactivity with a monoclonal antibody
Journal of Neurochemistry
A MarksR Baumal
Abstract
A hybridoma cell line producing a monoclonal antibody (A4) against bovine S100 protein has been produced by fusing mouse myeloma P3X63/Ag8 cells with spleen cells from a BALB/c mouse immunized with bovine S100 protein. A4 is of the IgG2b subclass and was purified by affinity chromatography on a protein A-Sepharose column. Brain extracts from several mammalian and one avian species reacted both with polyclonal rabbit anti-S100 protein antiserum and with A4 in a radioimmunoassay. Brain extract from dog was a notable exception. It reacted with the rabbit antiserum but not with A4. Therefore A4 reacts with a common epitope that is present on S100 proteins from different vertebrate species but is absent on dog S100 protein.
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Citations
May 31, 1993·Behavioural Brain Research·R GerlaiJ Roder
Mar 1, 1996·Brain Research. Molecular Brain Research·A MarksL E Becker
Aug 1, 1996·Physiology & Behavior·J K RoderR Gerlai
Mar 15, 2001·Brain Research. Developmental Brain Research·A G CoppensL Poncelet
Oct 24, 2002·Brain Research. Molecular Brain Research·Richard H DyckG Campbell Teskey
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L J Van EldikR A Jensen
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D BaileyA Marks
Dec 1, 1984·Journal of Bioenergetics and Biomembranes·E Zubrzycka-GaarnD H MacLennan
Nov 24, 2004·Molecular and Cellular Neurosciences·Jean Christophe DeloulmeJacques Baudier
Jun 6, 2000·Experimental Cell Research·Z XiongA Marks
Apr 1, 1991·Journal of Neuroscience Research·S KentrotiA Vernadakis
Aug 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Marks, R Allore
Mar 2, 2013·Biological & Pharmaceutical Bulletin·Jong Hoon WonDae Kyong Kim
Jun 1, 1986·Cell Calcium·R Donato
Nov 1, 1983·Biochimie·A MarksJ Mahony
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