PMID: 2107100Feb 26, 1990Paper

The structural elements of hirudin which bind to the fibrinogen recognition site of thrombin are exclusively located within its acidic C-terminal tail

FEBS Letters
J Y ChangJ M Schlaeppi

Abstract

Six lysyl residues of human thrombin (LysB21, LysB52, LysB65, LysB106, LysB107 and LysB154) have been previously shown to participate in the binding site of hirudin, a thrombin-specific inhibitor [(1989) J. Biol. Chem. 264, 7141-7146]. In this report, we attempted to delineate the region of hirudin which binds to these basic amino acids of thrombin. Using the N-terminal core domains (r-Hir1-43 and r-Hir1-52) derived from recombinant hirudins and synthetic C-terminal peptides (Hir40-65 and Hir52-65)--all fragments form complexes with thrombin--we are able to demonstrate that the structural elements of hirudin which account for the shielding of these 6 lysyl residues are exclusively located within the acidic C-terminal region. Since hirudin C-terminal peptides were shown to bind to a non-catalytic site of thrombin and inhibit its interaction with fibrinogen [(1987) FEBS Lett. 211, 10-16], our data consequently imply that these 6 lysyl residues are constituents of the fibrinogen recognition site of thrombin.

References

Jan 1, 1976·Methods in Enzymology·D BagdyS Magnusson
May 1, 1988·Analytical Biochemistry·J Y Chang
Aug 12, 1986·Biochemistry·S R Stone, J Hofsteenge
Dec 15, 1986·The Biochemical Journal·J Y Chang
Apr 1, 1985·Biological Chemistry Hoppe-Seyler·J DodtH Fritz
Jan 1, 1983·Methods in Enzymology·M W HunkapillerL E Hood
Jan 1, 1981·Annals of the New York Academy of Sciences·J W Fenton

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Citations

Aug 1, 1996·Journal of Protein Chemistry·T Kikuchi
Sep 20, 1991·Journal of Molecular Biology·T J RydelR Huber
Jan 19, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·H D MenssenE Thiel
Apr 23, 1991·Biochemistry·S R Stone, J Hofsteenge
Jun 13, 2019·Parasitology Research·Phil LukasChristian Müller
Aug 1, 1996·International Journal of Peptide and Protein Research·R MuramatsuH Hayashi
Aug 19, 1991·FEBS Letters·U B RasmussenE Van Obberghen-Schilling
Jan 31, 1997·Biophysical Chemistry·E Zhang, A Tulinsky
Jan 1, 1993·Thrombosis Research·M T Stubbs, W Bode
Jun 1, 1991·Thrombosis Research·J M Schlaeppi

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