The structural genes for three Drosophila glue proteins reside at a single polytene chromosome puff locus.

Molecular and Cellular Biology
T E CrowleyE M Meyerowitz

Abstract

The polytene chromosome puff at 68C on the Drosophila melanogaster third chromosome is thought from genetic experiments to contain the structural gene for one of the secreted salivary gland glue polypeptides, sgs-3. Previous work has demonstrated that the DNA included in this puff contains sequences that are transcribed to give three different polyadenylated RNAs that are abundant in third-larval-instar salivary glands. These have been called the group II, group III, and group IV RNAs. In the experiments reported here, we used the nucleotide sequence of the DNA coding for these RNAs to predict some of the physical and chemical properties expected of their protein products, including molecular weight, amino acid composition, and amino acid sequence. Salivary gland polypeptides with molecular weights similar to those expected for the 68C RNA translation products, and with the expected degree of incorporation of different radioactive amino acids, were purified. These proteins were shown by amino acid sequencing to correspond to the protein products of the 68C RNAs. It was further shown that each of these proteins is a part of the secreted salivary gland glue: the group IV RNA codes for the previously described sgs-3, whereas the g...Continue Reading

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Citations

May 1, 1990·Developmental Biology·M RoarkE M Meyerowitz
Mar 1, 1984·Developmental Biology·T E Crowley, E M Meyerowitz
May 20, 1988·Journal of Molecular Biology·C H MartinE M Meyerowitz
Mar 1, 1988·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Paul David ShirkChee Hark Harn
Jan 1, 1989·Developmental Genetics·D A KimbrellJ W Fristrom
Aug 1, 1988·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C H Martin, E M Meyerowitz
Jul 1, 1993·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·P S CoelhoM L Paçó-Larson
Jun 20, 2019·Bioinspiration & Biomimetics·Denisa Beňová-LiszekováRobert Farkaš
Jun 1, 1996·Genome Génome / Conseil National De Recherches Canada·E PardaliP Mavragani-Tsipidou
Jan 31, 2019·BMC Evolutionary Biology·Jean-Luc Da LageVirginie Courtier-Orgogozo
Jan 1, 1995·Insect Biochemistry and Molecular Biology·E SimmerlU Schäfer
Jul 20, 1986·Journal of Molecular Biology·E M Shore, G M Guild
Dec 1, 1986·Developmental Biology·M A Crosby, E M Meyerowitz
Aug 25, 1983·Journal of Molecular Biology·M D GarfinkelE M Meyerowitz

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