The structural organization of oligonucleosomes

Journal of Biomolecular Structure & Dynamics
C Marion

Abstract

We have used electric birefringence to study the structure of oligonucleosomes and to show the influence of histone H1 depletion on their conformation in solution. Measurements are made at low ionic strength on monodisperse samples containing up to 8 nucleosomes. For each oligomer, having H1 or not, the analysis of both relaxation and orientation times gives information about the particle's orientation mechanism through the ratio r of permanent over induced dipole terms. For native oligomers, the data confirm the previous finding of a discontinuity in hydrodynamic behavior between pentamer and heptamer: the rotational times are multiplied by 10 and r increases from 0.2 to 0.7 showing the appearance of a non-negligible contribution of a permanent dipole to the orientation mechanism. We suggest a model for the hexanucleosome at low ionic strength and discuss its implications for the higher-order structure of chromatin. The treatment for H1 depletion abolishes the transitions in electro-optical properties: the value of r remains constant, r = 0.15, and both rotational times increase progressively with the number of nucleosomes in the chain. That reflects an important unfolding of oligonucleosomal structure which we attributed to t...Continue Reading

References

Sep 1, 1977·Cell·A Worcel, C Benyajati
May 1, 1978·Nucleic Acids Research·A M CampbellJ F Pardon
Nov 1, 1978·Nucleic Acids Research·C Marion, B Roux
Nov 22, 1976·Biochemical and Biophysical Research Communications·B R Shaw, K S Schmitz
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J T Finch, A Klug
May 15, 1973·Biochemical and Biophysical Research Communications·D R Hewish, L A Burgoyne
Aug 1, 1973·The Review of Scientific Instruments·J C BernengoM Hanss
Jun 15, 1973·European Journal of Biochemistry·L A Bolund, E W Johns
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley
May 27, 1982·Nature·S Weisbrod
Jan 1, 1983·CRC Critical Reviews in Biochemistry·P J Butler
Jan 1, 1982·CRC Critical Reviews in Biochemistry·I L CartwrightG C Howard
Jul 1, 1983·European Journal of Biochemistry·V L MakarovP T Petrov
Oct 29, 1982·Biochemical and Biophysical Research Communications·C MarionB Roux
Mar 17, 1981·Biochemistry·K S LeeD M Crothers
Nov 1, 1981·European Journal of Biochemistry·C MarionJ C Bernengo
Jul 15, 1980·Journal of Molecular Biology·P J Butler, J O Thomas

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Citations

Jan 1, 1985·Chromosoma·J A SubiranaJ Frank
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·S H LeubaC Bustamante
Apr 14, 1995·The Journal of Biological Chemistry·K van Holde, J Zlatanova
Feb 1, 1992·Quarterly Reviews of Biophysics·B NordenT Kurucsev
Apr 1, 1988·Journal of Biomolecular Structure & Dynamics·S I DimitrovV L Makarov
Aug 1, 1990·Journal of Biomolecular Structure & Dynamics·M KubistaB Nordén
Aug 1, 1990·Journal of Biomolecular Structure & Dynamics·S I DimitrovI G Pashev
Oct 1, 1990·Journal of Biomolecular Structure & Dynamics·M F Hacques, C Marion
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Limeng ZhouDianjing Guo

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