The Structural Versatility of the BTB Domains of KCTD Proteins and Their Recognition of the GABAB Receptor

Biomolecules
Nicole BalascoLuigi Vitagliano

Abstract

Several recent investigations have demonstrated that members of the KCTD (Potassium Channel Tetramerization Domain) protein family are involved in fundamental processes. However, the paucity of structural data available on these proteins has frequently prevented the definition of their biochemical role(s). Fortunately, this scenario is rapidly changing as, in very recent years, several crystallographic structures have been reported. Although these investigations have provided very important insights into the function of KCTDs, they have also raised some puzzling issues. One is related to the observation that the BTB (broad-complex, tramtrack, and bric-à-brac) domain of these proteins presents a remarkable structural versatility, being able to adopt a variety of oligomeric states. To gain insights into this intriguing aspect, we performed extensive molecular dynamics simulations on several BTB domains of KCTD proteins in different oligomeric states (monomers, dimers, tetramers, and open/close pentamers). These studies indicate that KCTD-BTB domains are stable in the simulation timescales, even in their monomeric forms. Moreover, simulations also show that the dynamic behavior of open pentameric states is strictly related to thei...Continue Reading

References

Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·S ZollmanF A Laski
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Mar 30, 2000·Protein Science : a Publication of the Protein Society·S KumarR Nussinov
Nov 17, 2004·Trends in Biochemical Sciences·Peter J Stogios, Gilbert G Privé
Oct 7, 2005·Genome Biology·Peter J StogiosGilbert G Privé
Oct 8, 2005·Journal of Computational Chemistry·David Van Der SpoelHerman J C Berendsen
Apr 25, 2007·Annals of Neurology·Patrick Van BogaertMarc J Abramowicz
Apr 14, 2009·Journal of Molecular Biology·Irina S DementievaSteve A N Goldstein
Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Valeria E Zarelli, Igor B Dawid
Apr 18, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mikhail SkoblovAncha Baranova
Sep 3, 2013·Journal of Molecular Recognition : JMR·Stefania CorrealeEmilia Pedone
Nov 26, 2013·Cell & Bioscience·Zhepeng LiuGuihong Sun
Apr 22, 2014·Biochimica Et Biophysica Acta·Nicole BalascoLuigi Vitagliano
Apr 8, 2015·PloS One·Ivan de PaolaLaura Zaccaro
May 15, 2015·PloS One·Giovanni SmaldoneLuigi Vitagliano
Sep 4, 2015·Journal of Molecular Biology·Alan X JiGilbert G Privé
Sep 5, 2015·Journal of Biomolecular Structure & Dynamics·Daniela BaroneLuigi Vitagliano
Feb 26, 2016·ACS Chemical Biology·Rafael MolinaMarco D'Abramo
Dec 23, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Thorsten FritziusBernhard Bettler
May 31, 2017·PLoS Genetics·Qiuling LiNicholas Stavropoulos
Oct 1, 2017·The Biochemical Journal·Daniel M PinkasAlex N Bullock
Nov 2, 2017·Nature·Christine Ochoa EscamillaCraig M Powell
Apr 18, 2018·International Journal of Biological Macromolecules·Giovanni SmaldoneEmilia Pedone
May 15, 2018·International Journal of Biological Macromolecules·Nicole BalascoLuigi Vitagliano
Apr 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Hao ZuoQing R Fan
Jun 15, 2019·CNS Neuroscience & Therapeutics·Xinchen TengJ Marie Hardwick

❮ Previous
Next ❯

Citations

Jan 30, 2021·Scientific Reports·Enrico GalloAntonella Accardo

❮ Previous
Next ❯

Methods Mentioned

BETA
ubiquitination

Software Mentioned

GROMACS
VMD

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.