The structure and function of p55PIK reveal a new regulatory subunit for phosphatidylinositol 3-kinase.

Molecular and Cellular Biology
S PonsM F White

Abstract

Phosphatidylinositol 3-kinase (PI-3 kinase) is implicated in the regulation of diverse cellular processes, including insulin-stimulated glucose transport. PI-3 kinase is composed of a 110-kDa catalytic subunit and an 85-kDa regulatory subunit. Here, we describe p55PIK, a new regulatory subunit that was isolated by screening expression libraries with tyrosine-phosphorylated insulin receptor substrate 1 (IRS-1). p55PIK is composed of a unique 30-residue NH2 terminus followed by a proline-rich motif and two Src homology 2 (SH2) domains with significant sequence identify to those in p85. p55PIK mRNA is expressed early during development, remains abundant in adult mouse brain and testis tissue, and is detectable in adult adipocytes and heart and kidney tissues. p55PIK forms a stable complex with p110, and it associates with IRS-1 during insulin stimulation. Moreover, the activated insulin receptor phosphorylates p55PIK in Sf9 cells, and insulin stimulates p55PIK phosphorylation in CHOIR/p55PIK cells. The unique features of p55PIK suggest that it is important in receptor signaling.

References

Nov 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G MyersM F White
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B MargolisJ Schlessinger
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N B RudermanL C Cantley
Mar 12, 1993·Cell·Z SongyangR J Lechleider
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L M ChuangC R Kahn
Jul 1, 1994·Trends in Biochemical Sciences·M G MyersM F White
Jul 29, 1994·Biochemical and Biophysical Research Communications·Y Shimizu, T Shimazu
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·K HaraT R Jackson
Aug 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Kapeller, L C Cantley
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C H LeeE Y Skolnik

❮ Previous
Next ❯

Citations

Mar 21, 2009·Trends in Biochemical Sciences·Klaartje KokBart Vanhaesebroeck
Mar 29, 2016·Brain Research·Tanja GüntertOmolara O Ogunshola
Sep 17, 2004·Biochemical and Biophysical Research Communications·Cristina M RondinoneJames M Trevillyan
Apr 22, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jiaojiao GuoRui-Ping Xiao
Jun 15, 2000·Experimental Cell Research·C SunshineK L Kirk
Jun 14, 2005·Biochimica Et Biophysica Acta·A Lulu StratRodney A Hill
Sep 15, 2004·Biochimica Et Biophysica Acta·Rodney A HillArieh Gertler
Mar 20, 2013·BioMed Research International·Yu DengJunbo Hu
Oct 6, 1998·Annual Review of Biochemistry·D A FrumanL C Cantley
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Katrine AlmindC Ronald Kahn
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·Kohjiro UekiLewis C Cantley
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Liliana SoroceanuHeidi S Phillips
May 15, 2007·Biology of Reproduction·Nir EtkovitzHaim Breitbart
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·A J MelendezJ M Allen
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B VanhaesebroeckM D Waterfield
Jun 25, 1999·Metabolism: Clinical and Experimental·M C SugdenH Lall
Mar 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·I M AparicioL J Garcia-Marin
Jan 27, 1998·The Journal of Biological Chemistry·J A GreenwoodJ E Murphy-Ullrich
Jul 30, 2009·Journal of Lipid Research·Raju V S Rajala
Apr 3, 2003·Immunological Reviews·Leonard J Appleman, Vassiliki A Boussiotis
Jun 27, 1997·The Journal of Biological Chemistry·D Sawka-VerhelleE Van Obberghen
Mar 29, 2005·Nature Cell Biology·Kathrine AbellChristine J Watson
Mar 12, 1998·Nature·D J WithersM F White
Dec 14, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Yogesh DwivediGhanshyam N Pandey
Jan 25, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Erik Procko, Shaun R McColl

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.