PMID: 7606779Jul 14, 1995

The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions

Cell
Z RaoDavid I Stuart

Abstract

Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. We have determined, at high resolution (1.5 A), the crystal structure of such a domain, from human clotting factor IX, as a complex with Ca2+. The Ca2+ ligands form a classic pentagonal bipyramid with six ligands contributed by one polypeptide chain and the seventh supplied by a neighboring EGF-like domain. The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains.

References

Nov 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·E Y JonesDavid I Stuart
May 9, 1991·Nature·Penny A HandfordG G Brownlee
Sep 21, 1990·Science·C CoffmanC Kintner
Apr 14, 1989·Biochemical and Biophysical Research Communications·L H HuangJ P Tam
Mar 24, 1995·The Journal of Biological Chemistry·Penny A HandfordCay M Kielty
Jan 1, 1993·Protein Engineering·G J Barton
May 1, 1995·Acta Crystallographica. Section D, Biological Crystallography·Z RaoDavid I Stuart
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·Collaborative Computational Project, Number 4

Citations

Dec 6, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J M ArnoldM F Lavin
Jan 1, 1997·Protein Science : a Publication of the Protein Society·S GillisM D Bond
Nov 5, 1999·Biochemical and Biophysical Research Communications·S KurodaKang Ting
Dec 22, 1999·Biochemical and Biophysical Research Communications·S Kuroda, K Tanizawa
Sep 29, 2005·Springer Seminars in Immunopathology·Rikke SørensenJens C Jensenius
May 26, 2005·Journal of Molecular Biology·Amy E SchmidtS P Bajaj
Jun 20, 2003·International Journal for Parasitology·David M WitcombeNicholas C Smith
Sep 28, 1999·Life Sciences·S B CatrinaV Mutt
Mar 1, 1996·Trends in Neurosciences·C W OlanowT B Freeman
Jan 14, 1998·Biochimica Et Biophysica Acta·J FangJ Bonadio
Aug 24, 2001·Domestic Animal Endocrinology·J G KimR K Christenson
Nov 9, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·Penny A HandfordL Y Sakai
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·P Maurer, E Hohenester
Jan 29, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·S SinhaCay M Kielty
Oct 1, 1996·Current Opinion in Cell Biology·P MaurerJ Engel
Aug 6, 1998·Insect Biochemistry and Molecular Biology·T W Sappington, A S Raikhel
Jan 30, 2003·Trends in Cardiovascular Medicine·Amy E Schmidt, S P Bajaj
Jul 17, 1998·Current Opinion in Chemical Biology·J EvenäsS Forsén
May 1, 1996·Quarterly Reviews of Biophysics·P BorkI D Campbell
Nov 15, 2002·Clinical Genetics·L TotoM Romano
Jan 1, 1996·Nature Structural Biology·E HohenesterJ Engel
Jul 29, 2008·Nature Structural & Molecular Biology·Jemima CordlePenny A Handford
Nov 4, 2000·Pediatrics International : Official Journal of the Japan Pediatric Society·M R OhD K Jin
Feb 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hyock Joo KwonJohann Deisenhofer
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Carsten ReissnerMarkus Missler
Jul 21, 2009·The Journal of Biological Chemistry·Richard P O JonesDorothy Trump
Sep 1, 1996·The Journal of Experimental Medicine·J HamannR A van Lier
May 9, 2006·Antioxidants & Redox Signaling·P WhitemanPenny A Handford
May 19, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas SchürpfJia-Huai Wang
Jan 14, 2000·Journal of Medical Genetics·P N Robinson, Maurice Godfrey
Jun 2, 2012·Frontiers in Bioscience (Landmark Edition)·Kanagasabai Vadivel, S P Bajaj
Mar 19, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Anbi Xu, Laiqiang Huang
Aug 31, 2001·Biochemical and Biophysical Research Communications·K E M PerssonJ Stenflo
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·C D DickinsonW Ruf
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Elise HennebertPatrick Flammang
Aug 26, 2014·Brain Research·Xiaojie ZhangMichael M Wang
Nov 24, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·F VellaG J Arlaud
Feb 10, 2012·Journal of the American Medical Informatics Association : JAMIA·Thomas A PetersonMaricel G Kann
Sep 5, 2014·Glycoconjugate Journal·Deepika Vasudevan, Robert S Haltiwanger
Jun 1, 1996·Current Opinion in Genetics & Development·F Ramirez
Oct 12, 2001·Mutation Research·S S SommerK A Hill
Jul 12, 2012·Cellular and Molecular Life Sciences : CMLS·Robert J Huber, Danton H O'Day
Mar 1, 2016·Frontiers in Oncology·Shanmugasundaram PakkiriswamiMarios Georgiou
Oct 27, 2015·International Journal of Molecular Medicine·Yichun WangJunming Luo
Jun 10, 2005·FEBS Letters·Raimund WagenerMats Paulsson
Jan 28, 2012·Biochemical and Biophysical Research Communications·Yuji KuriharaKohtaro Takei
Apr 13, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Mahroo K ParsiMark A Gibson
Oct 28, 2008·The International Journal of Biochemistry & Cell Biology·Kelvin B Luther, Robert S Haltiwanger
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·L AutinBruno O Villoutreix
Sep 29, 2015·Nature Chemical Biology·Hongjun YuHideyuki Takeuchi
Jan 2, 2007·Biochemical and Biophysical Research Communications·Eun Mi HwangJae-Yong Park
Aug 15, 2006·Biochemical and Biophysical Research Communications·M PreisM Y Flugelman
May 27, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Michael J FitchHeidi Stuhlmann
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·Karin E KnobeRolf C R Ljung
Sep 1, 2005·Journal of Thrombosis and Haemostasis : JTH·K Hansson, J Stenflo
Jan 31, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shawn BeugCatherine Tsilfidis
Dec 30, 2014·Molecules and Cells·Dong Yeol KimByung Ju Lee
Aug 19, 2015·Mutation Research. Reviews in Mutation Research·Karina A Zeyer, D P Reinhardt
Jul 19, 2014·Expert Opinion on Drug Delivery·Estrella Lopez-GordoAndrew H Baker
Oct 17, 2013·Insect Biochemistry and Molecular Biology·Alexander D Smith, W Reuben Kaufman
Dec 8, 2000·Biochimica Et Biophysica Acta·Penny A Handford
Jun 26, 2012·Journal of Molecular Biology·Yingnan ZhangDaniel Kirchhofer
Nov 26, 2009·Biochemical and Biophysical Research Communications·Dong-Gyu KimJae-Yong Park
Feb 3, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·Ulf Ryde
Jun 16, 2009·Protein Expression and Purification·Srdjan PicuricStefanie Oess
Jul 29, 2010·Protist·Leila M BlackmanAdrienne R Hardham
May 22, 2013·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Xabier Arias-MorenoJose F Lucía
Nov 3, 2000·Human Mutation·M L HeritageG J Anderson
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·S H Shiu, A B Bleecker
Feb 20, 1999·Journal of Molecular Biology·R SmallridgeA K Downing
Mar 21, 2007·Insect Biochemistry and Molecular Biology·Robert D MitchellR Michael Roe
Sep 12, 1997·The Journal of Biological Chemistry·P McCloskeyE T Liu
Jun 16, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Huizhen WangDaishu Han
Feb 2, 2018·Journal of Cellular Biochemistry·Huaxiang ZhaoFeng Chen
Dec 1, 1996·Circulation·D M MilewiczA Biddinger
Nov 28, 2018·Nanoscale·Nadine KampradMarco Tarantola
Nov 30, 2018·Glycobiology·Florian PennarubiaSébastien Legardinier
Jan 19, 2007·Pathophysiology of Haemostasis and Thrombosis·Karin E KnobeRolf C R Ljung
Aug 12, 1999·Annals of Medicine·F RamirezL Pereira
Jul 22, 2017·The Journal of Biological Chemistry·Hideyuki TakeuchiRobert S Haltiwanger
Sep 10, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T-M FungA M Smith
Jan 10, 1997·The Journal of Biological Chemistry·D P ReinhardtL Y Sakai
Sep 25, 2002·The Journal of Biological Chemistry·Ruey-Bing YangJames N Topper
Jun 6, 2003·The Journal of Biological Chemistry·Lynn A GregoryChristine Gaboriaud
Jun 11, 2020·Glycobiology·Florian PennarubiaSébastien Legardinier
Aug 12, 1996·FEBS Letters·M J Hubbard, N J McHugh
Mar 1, 2017·Drug Delivery and Translational Research·Sarena D Horava, Nicholas A Peppas
Jun 30, 2019·BMC Bioinformatics·Lennon Meléndez-ArandaMarina María de Jesús Romero-Prado
Mar 14, 1997·The Journal of Biological Chemistry·D P ReinhardtH P Bächinger
Apr 23, 2008·Current Protocols in Protein Science·Peter D SunJeffrey C Boyington
Nov 14, 1998·The American Journal of Physiology·Y S KanwarD R Peterson
May 29, 1998·European Journal of Immunology·J HamannR A van Lier
Jul 24, 2020·Scientific Reports·Synnøve Norvoll MagnussenGunbjørg Svineng
Aug 16, 1996·The Journal of Biological Chemistry·Z H HeB D Kohorn
Feb 7, 2003·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·K E M Persson
Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·I CaminschiM D Wright
Aug 24, 2013·International Journal of Oncology·Michael SafaeeAndrew T Parsa
Mar 1, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·J F HernandezG J Arlaud
Jul 13, 2000·The Journal of Biological Chemistry·H Jeon, G G Shipley
Jun 11, 2019·Frontiers in Physiology·Robert D MitchellAdalberto A Pérez de León
Oct 8, 2020·Annals of Human Genetics·Craig C TeerlinkLisa A Cannon-Albright

Related Concepts

Calcium
Computer Graphics
Epidermal Growth Factor
Factor IX
Protein Conformation
Determination, Sequence Homology
Crystallography, X-Ray

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.