The structure of a local population of phytopathogenic Pseudomonas brassicacearum from agricultural soil indicates development under purifying selection pressure

Environmental Microbiology
J SikorskiW Wackernagel

Abstract

Among the isolates of a bacterial community from a soil sample taken from an agricultural plot in northern Germany, a population consisting of 119 strains was obtained that was identified by 16S rDNA sequencing and genomic fingerprinting as belonging to the recently described species Pseudomonas brassicacearum. Analysis of the population structure by allozyme electrophoresis (11 loci) and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR; four primers) showed higher resolution with the latter method. Both methods indicated the presence of three lineages, one of which dominated strongly. Stochastic tests derived from the neutral theory of evolution (including Slatkin's exact test, Watterson's homozygosity test and the Tajima test) indicated that the population had developed under strong purifying selection pressure. The presence of strains clearly divergent from the majority of the population can be explained by in situ evolution or by influx of strains as a result of migration or both. Phytopathogenicity of a P. brassicacearum strain determined with tomato plants reached the level obtained with the type strain of the known pathogen Pseudomonas corrugata. The results show that a selective sweep was identified ...Continue Reading

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Citations

Jan 22, 2010·Extremophiles : Life Under Extreme Conditions·David A PearceA E Jones
Aug 1, 2007·Journal of Industrial Microbiology & Biotechnology·Muhammad SaleemAhmad Saeed Bhatti
Oct 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Johannes Sikorski, Eviatar Nevo
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Jun 27, 2017·Phytopathology·Daniel SchlatterTimothy Paulitz
Oct 1, 2013·Plant Disease·Cheng-Hua HuangDavid J Norman

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