PMID: 6168999Jun 11, 1981Paper

The structure of a thirty-six kilobase region of the human chromosome including the fibroblast interferon gene IFN-beta

Nucleic Acids Research
G GrossJ Collins

Abstract

The isolation of a human genomic cosmid hybrid containing the interferon beta gene has recently been reported (Gross et al., 1981). This hybrid was mapped using single and double digests and cross-hybridisation with the sub-cloned EcoRI and BgIII fragments. Purified fragments and subclones were used as hybridisation probes against chromosomal "Southern" blots to show that at least half of the region has been cloned without alteration. This cannot at present be confirmed for the rest of the region due to the presence of highly repetitive DNA on these cloned fragments. Sequencing data on the 5'-end of the fibroblast IFN-beta gene shows a high density of direct and inverted repeats. The IFN-beta mRNA coding region contains no intron, although the possibility of other transcription starts is not ruled out. The cloned region shows no similarities to known genomic clones containing IFN-alpha genes.

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Citations

Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K ZinnT Maniatis
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Canaani, P Berg
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M OhlssonA von Gabain
Sep 1, 1982·The Journal of Experimental Medicine·A D SagarF H Ruddle
Jan 1, 1985·Journal of Interferon Research·K A KelleyP M Pitha
Jun 1, 1993·Journal of Interferon Research·M O DiazG Allen
Dec 1, 1993·Journal of Interferon Research·M O DiazG Allen
Feb 1, 1993·Journal of Interferon Research
Aug 1, 1994·Journal of Interferon Research·M O DiazG Allen
Feb 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M O DiazG Allen
Feb 11, 1985·Nucleic Acids Research·E C ZwarthoffJ Trapman
Jun 15, 1983·European Journal of Biochemistry·J ShuttleworthD C Burke
Jan 1, 1985·Journal of Interferon Research·N B RajP M Pitha
Sep 30, 1982·Biochimica Et Biophysica Acta·P B Sehgal
Nov 1, 1994·Molecular and Cellular Biology·H M PomykalaM O Díaz

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