PMID: 236092Mar 1, 1975

The structure of capsular polysaccharide of the Pneumococcus type II

Carbohydrate Research
L KenneS Svensson

Abstract

The pneumococcus type II capsular polysaccharide (SII) is composed of singly-branched hexasaccharide repeating units, for which three alternative structures have been proposed. The correct structure has now been determined by consecutive eliminations of the sugar residues in the side chain. The terminal D-glucuronic acid group was eliminated by treating the fully methylated and esterified SIIpolysaccharide first with base, and then with weak acid. The hydroxyl group at C-6 in the penultimate D-glucose residue released by this elimination was transformed into the 6-deoxy-6-tosyl derivative, and the residue thereafter eliminated by treatment with base. As the side-chains were eliminated by these reactions, it is considered that they contain only two sugar residues, which thus excludes two of the three alternative structures. Structure 1 was further confirmed by subjecting SII to a Smith degradation, which yielded the tetrasaccharide L-Rhap-(1 yields 3)-L-Rhap-(1 yields 3)-L-Rhap-(1 yields 2-erythritol, characterised by methylation analysis.

References

Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M HEIDELBERGER, A W Bernheimer
Oct 1, 1985·The Journal of Experimental Medicine·M HEIDELBERGERB Jann
Oct 3, 2006·Biochemistry. Biokhimii︠a︡·Yu S Ovodov
Apr 10, 2020·Organic & Biomolecular Chemistry·Diksha Rai, Suvarn S Kulkarni
Apr 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·M HEIDELBERGERS Stirm

Related Concepts

Rhamnose, L-Isomer
Erythritol
Protein Methylation
Methylate
Glucose, (beta-D)-Isomer
Polysaccharides, Bacterial
Mass Spectrometry
D-GLUCURONIC ACID
Methylation
Excretory Function

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.