The structure of cucumber mosaic virus: cryoelectron microscopy, X-ray crystallography, and sequence analysis

Virology
W R WikoffJ E Johnson

Abstract

The three-dimensional structure of cucumber mosaic virus (CMV) was analyzed at 23 A resolution by cryoelectron microscopy and image reconstruction, demonstrating structural similarity to cowpea chlorotic mottle virus (CCMV), another member of the Bromoviridae family. The CMV structure was determined at 8 A resolution by X-ray crystallography with phases determined by single isomorphous replacement and refined by fivefold noncrystallographic symmetry averaging. The X-ray structure agreed with the electron microscopy reconstruction; the electron density is consistent with beta-barrel subunits arranged with T = 3 quasi-symmetry in an orientation similar to that observed in CCMV. Strong density surrounding the icosahedral threefold axes (quasi sixfold axes in the T = 3 particle) between 80 and 100 A from the particle center formed a cylinder of radius 11 A, similar to the density observed in the same region of CCMV. This density corresponds to the beta-annulus of CCMV, which differentiates hexamers from pentamers and determines the formation of the T = 3 particles. The CMV and CCMV amino acid sequences were aligned, providing information (based on the CCMV atomic model) about the probable distribution of residues in the three-dimen...Continue Reading

References

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Citations

Jun 23, 2000·Journal of Virology·P J GlassM K Estes
Jun 11, 2002·Journal of Molecular Biology·Florence Tama, Charles L Brooks
Mar 30, 2004·Journal of Molecular Biology·Ricardo A BernalMichael G Rossmann
Oct 31, 2002·Journal of Structural Biology·Robert W LucasAlexander McPherson
Dec 5, 1998·Virology·F OsmanA L Rao
Apr 11, 2008·Chemical Communications : Chem Comm·Yun XuRongxiang Fang
Mar 17, 2018·Biochemistry. Biokhimii︠a︡·G K SelikhanovA G Gabdulkhakov

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