The structure of HIV-1 genomic RNA in the gp120 gene determines a recombination hot spot in vivo.

The Journal of Biological Chemistry
Román GalettoMatteo Negroni

Abstract

By frequently rearranging large regions of the genome, genetic recombination is a major determinant in the plasticity of the human immunodeficiency virus type I (HIV-1) population. In retroviruses, recombination mostly occurs by template switching during reverse transcription. The generation of retroviral vectors provides a means to study this process after a single cycle of infection of cells in culture. Using HIV-1-derived vectors, we present here the first characterization and estimate of the strength of a recombination hot spot in HIV-1 in vivo. In the hot spot region, located within the C2 portion of the gp120 envelope gene, the rate of recombination is up to ten times higher than in the surrounding regions. The hot region corresponds to a previously identified RNA hairpin structure. Although recombination breakpoints in vivo cluster in the top portion of the hairpin, the bias for template switching in this same region appears less marked in a cell-free system. By modulating the stability of this hairpin we were able to affect the local recombination rate both in vitro and in infected cells, indicating that the local folding of the genomic RNA is a major parameter in the recombination process. This characterization of reve...Continue Reading

References

Jun 14, 1967·Journal of Molecular Biology·B Hirt
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M NegroniH Buc
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·J K YeeT Friedmann
Mar 30, 1999·Biochimica Et Biophysica Acta·B I KlasensB Berkhout
Jan 11, 2000·Journal of Virology·A E JetztJ P Dougherty
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Negroni, H Buc
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·M WisniewskiR A Bambara
Jul 4, 2001·The Journal of Biological Chemistry·M BalakrishnanR A Bambara
Sep 15, 2001·Nucleic Acids Research·A MoumenM Negroni
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·C K HwangV K Pathak
Oct 9, 2002·The Journal of Biological Chemistry·Ricardo H RodaRobert A Bambara
Oct 22, 2002·Journal of Virology·Jianling ZhuangJoseph P Dougherty
Jan 14, 2003·Annual Review of Immunology·Daniel C DouekRichard A Koup
Jan 21, 2003·The Journal of Biological Chemistry·Aarti Raja, Jeffrey J DeStefano
Feb 22, 2003·The Journal of Biological Chemistry·Abdeladim MoumenMatteo Negroni
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Jan 7, 2004·Nature Reviews. Genetics·Andrew RambautEdward C Holmes
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·David N LevyGeorge M Shaw

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Citations

Jul 9, 2011·The Journal of Biological Chemistry·Wen ShenRobert A Bambara
Oct 22, 2009·AIDS Research and Human Retroviruses·Leandro R JonesManuel Gomez-Carrillo
Sep 28, 2006·Nucleic Acids Research·Heather A BairdMatteo Negroni
Oct 6, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Mia CoetzerDonald E Mosier
Oct 1, 2010·Journal of Virology·Etienne Simon-LoriereMatteo Negroni
Feb 14, 2006·Journal of Virology·Christian Lanciault, James J Champoux
Sep 13, 2008·PLoS Computational Biology·John ArcherDavid L Robertson
Oct 14, 2011·Viruses·Krista Delviks-FrankenberryWei-Shau Hu
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mario P S ChinWei-Shau Hu
Apr 25, 2012·Journal of Neurovirology·Jun Yong ChoiUNKNOWN Charter Group
Sep 26, 2014·Viruses·Jonathan M O Rawson, Louis M Mansky
Jul 15, 2015·Virology·Olga NikolaitchikWei-Shau Hu
Mar 1, 2008·Virus Research·Bertha Cecilia RamirezMatteo Negroni
Oct 18, 2015·Nucleic Acids Research·Zsuzsanna SükösdJørgen Kjems
Oct 10, 2015·Nature Communications·Ron GellerRafael Sanjuán
Oct 10, 2006·Comptes rendus biologies·Maurice L J MoncanyPascal R R Courtois
Apr 28, 2005·Journal of Molecular Biology·Igor KanevskyPhilippe Fossé
Sep 14, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jun FanDavid L Robertson
Oct 31, 2007·PLoS Computational Biology·Gajendra W Suryavanshi, Narendra M Dixit
Oct 23, 2016·Cellular and Molecular Life Sciences : CMLS·Rafael Sanjuán, Pilar Domingo-Calap
Oct 12, 2016·Viruses·Lizna M AliFarah Mustafa
Dec 29, 2013·Journal of Virology·Redmond P SmythMiles P Davenport
Jan 24, 2014·Journal of Virology·Timothy E SchlubMiles P Davenport
Nov 16, 2004·The Journal of Biological Chemistry·Christian Lanciault, James J Champoux
Mar 6, 2007·The Journal of Biological Chemistry·Vandana PurohitRobert A Bambara
Dec 17, 2010·RNA Biology·Judith G LevinKarin Musier-Forsyth
Nov 18, 2005·The Journal of Biological Chemistry·Román GalettoMatteo Negroni

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