The structure of OmpF porin in a tetragonal crystal form

Structure
S W CowanJ P Rosenbusch

Abstract

OmpF porin is a trimeric integral membrane protein responsible for the passive transport of small hydrophilic molecules, such as nutrients and waste products, across the outer membrane of Escherichia coli. Very few membrane proteins have been crystallized in three dimensions, yet this stable protein can be obtained in several crystal forms. Comparison of the structures of the same membrane protein in two different packing environments is of major interest, because it allows us to explore the integrity of the structure outside the natural membrane environment. The structure of OmpF porin in a tetragonal crystal form with two trimers per asymmetric unit has been determined at 3.2 A resolution and compared with that obtained previously in a trigonal crystal form. The lattice contacts involve only polar atoms, whereas extensive hydrophobic protein-protein interactions were found in the trigonal lattice. The trimer structure is virtually identical in both. Our comparison reveals that the overall structure of OmpF is not influenced by crystal lattice constraints and, thus, presumably bears close resemblance to the in vivo structure. The tetragonal crystal structure has provided the starting model for the phasing of neutron diffractio...Continue Reading

References

Nov 16, 1992·Science·H Nikaido, M H Saier
Sep 20, 1992·Journal of Molecular Biology·M S Weiss, G E Schulz
Mar 25, 1991·FEBS Letters·M S WeissG E Schulz
Oct 1, 1991·Journal of Structural Biology·R A PauptitG E Schultz
Jan 5, 1991·Journal of Molecular Biology·A KreuschG E Schulz
Apr 5, 1991·Journal of Molecular Biology·R A PauptitJ P Rosenbusch
Jan 20, 1990·Journal of Molecular Biology·K A StaufferR A Pauptit
Nov 1, 1990·Quarterly Reviews of Biophysics·B K Jap, P J Walian
Jul 23, 1984·FEBS Letters·M Schindler, J P Rosenbusch
Jul 1, 1980·The Journal of Cell Biology·R M Garavito, J P Rosenbusch
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H Schindler, J P Rosenbusch
Jul 22, 1994·Journal of Molecular Biology·A KarshikoffT Schirmer
Jan 1, 1994·Protein Science : a Publication of the Protein Society·A KreuschG E Schulz
Aug 1, 1993·Current Opinion in Cell Biology·G E Schulz
Oct 15, 1995·Structure·E Pebay-PeyroulaP A Timmins
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

❮ Previous
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Citations

Jul 16, 2005·Die Naturwissenschaften·John KatsarasMu-Ping Nieh
Aug 30, 2008·European Biophysics Journal : EBJ·Catalin ChimerelUlrich Kleinekathöfer
Dec 29, 2012·European Biophysics Journal : EBJ·Usha LamichhaneMathias Winterhalter
Apr 6, 2004·Current Opinion in Microbiology·Sarah E Ades
Sep 12, 2000·Current Opinion in Structural Biology·M Caffrey
Apr 13, 1999·Bioorganic & Medicinal Chemistry·L P Liu, C M Deber
Jul 20, 2000·Micron : the International Research and Review Journal for Microscopy·J P RosenbuschM Regenass
Apr 30, 2003·Journal of Structural Biology·Martin Caffrey
Jan 5, 2002·Journal of Molecular Graphics & Modelling·F BasynR Brasseur
Oct 26, 2002·Journal of Molecular Graphics & Modelling·Jeffrey Sutherland, Gustavo A Arteca
Oct 6, 2012·Chemical Reviews·Christopher MaffeoAleksei Aksimentiev
Oct 11, 2013·Journal of the American Chemical Society·Balasundaresan DhakshnamoorthyBenoît Roux
Sep 26, 2008·The Journal of Physical Chemistry. B·Yiming MoWilliam T Heller
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·E M Landau, J P Rosenbusch
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·P S PhaleJ P Rosenbusch
Dec 30, 2010·The Journal of Biological Chemistry·Mohammad M MohammadLiviu Movileanu
Oct 2, 2013·The Journal of General Physiology·Fatemeh Khalili-AraghiBenoît Roux
Aug 23, 2002·Protein Science : a Publication of the Protein Society·Yufeng Zhai, Milton H Saier
Jan 16, 2002·Protein Science : a Publication of the Protein Society·William C Wimley
Mar 29, 2001·Protein Science : a Publication of the Protein Society·I JacoboniR Casadio
Oct 2, 2007·Journal of Bacteriology·Melissa PagelAnne H Delcour
Jun 11, 2004·Annual Review of Biochemistry·Vassilis KoronakisColin Hughes
Apr 29, 1998·Annual Review of Physiology·K Kirk, K Strange
Aug 23, 2011·Journal of Nanobiotechnology·Manuel KrewinkelMarco Fioroni
Sep 24, 1999·Biological Chemistry·A WieseS Müller-Loennies
Jun 2, 2011·Biological Chemistry·Marco LolicatoVito De Pinto
Sep 26, 2012·Biochemistry Research International·Elena García-GiménezVicente M Aguilella
Apr 7, 2004·Médecine sciences : M/S·Jean-Marie Pagès
Feb 20, 2016·Journal of Molecular Graphics & Modelling·Jitti NiramitranonPrapasiri Pongprayoon
Nov 25, 2003·FEBS Letters·Vassilis Koronakis
Dec 19, 2015·Journal of the American Chemical Society·Steven S KlaraMeagan S Mauter
Jan 2, 2016·Biochimica Et Biophysica Acta·Karunakar R PothulaUlrich Kleinekathöfer
Apr 25, 2012·Journal of Structural Biology·D BalasubramaniamS Krishnaswamy
Apr 10, 2012·Journal of Structural Biology·Rouslan G Efremov, Leonid A Sazanov

❮ Previous
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