The structure of serine hydroxymethyltransferase as modeled by homology and validated by site-directed mutagenesis

Protein Science : a Publication of the Protein Society
S PascarellaF Bossa


We describe a model for the three-dimensional structure of E. coli serine hydroxymethyltransferase based on its sequence homology with other PLP enzymes of the alpha-family and whose tertiary structures are known. The model suggests that certain amino acid residues at the putative active site of the enzyme can adopt specific roles in the catalytic mechanism. These proposals were supported by analysis of the properties of a number of site-directed mutants. New active site features are also proposed for further experimental testing.


May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Mar 5, 1992·Nature·R LüthyD Eisenberg
May 20, 1992·Journal of Molecular Biology·C A McPhalenJ N Jansonius
Jan 14, 1992·Biochemistry·S AngelaccioV Schirch
Apr 25, 1991·Nucleic Acids Research·A Bairoch, B Boeckmann
Jan 1, 1990·Annals of the New York Academy of Sciences·G Joshi-Tope, V Schirch
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M GribskovD Eisenberg
Apr 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·H C Dunathan
Apr 15, 1984·Journal of Molecular Biology·J F KirschP Christen
Dec 25, 1981·Journal of Molecular Biology·J Greer
Mar 17, 1995·Journal of Molecular Biology·V N MalashkevichY M Torchinsky
Apr 27, 1993·Biochemistry·A A AntsonK S Wilson
May 20, 1994·Journal of Molecular Biology·I K McDonald, J M Thornton
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Jun 1, 1993·European Journal of Biochemistry·P K MehtaP Christen
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·M HennigJ N Jansonius
Jul 18, 1997·Journal of Molecular Biology·B RostC Sander

❮ Previous
Next ❯


Apr 13, 2000·The International Journal of Biochemistry & Cell Biology·N A RaoH S Savithri
Aug 21, 2013·BioMed Research International·Martino L Di SalvoH Tonie Wright
May 30, 2003·The Journal of Biological Chemistry·Tzu-Fun FuVerne Schirch

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.