The structure of SpnF, a standalone enzyme that catalyzes [4 + 2] cycloaddition

Nature Chemical Biology
Christopher D FageAdrian T Keatinge-Clay

Abstract

In the biosynthetic pathway of the spinosyn insecticides, the tailoring enzyme SpnF performs a [4 + 2] cycloaddition on a 22-membered macrolactone to forge an embedded cyclohexene ring. To learn more about this reaction, which could potentially proceed through a Diels-Alder mechanism, we determined the 1.50-Å-resolution crystal structure of SpnF bound to S-adenosylhomocysteine. This sets the stage for advanced experimental and computational studies to determine the precise mechanism of SpnF-mediated cyclization.

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Methods Mentioned

BETA
gel filtration
Assay

Software Mentioned

AutoDock Vina
Phaser
UFF
UCSF Chimera
RebM
Refmac5
DOCK
XDS
Dock Prep
DALI

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