The structure of the Escherichia coli phosphotransferase IIAmannitol reveals a novel fold with two conformations of the active site

Structure
R L van MontfortB W Dijkstra

Abstract

The bacterial phosphoenolpyruvate-dependent phosphotransferase system (PTS) catalyses the cellular uptake and subsequent phosphorylation of carbohydrates. Moreover, the PTS plays a crucial role in the global regulation of various metabolic pathways. The PTS consists of two general proteins, enzyme I and the histidine-containing protein (HPr), and the carbohydrate-specific enzyme II (EII). EIIs are usually composed of two cytoplasmic domains, IIA and IIB, and a transmembrane domain, IIC. The IIA domains catalyse the transfer of a phosphoryl group from HPr to IIB, which phosphorylates the transported carbohydrate. Knowledge of the structures of the IIA proteins may provide insight into the mechanisms by which the PTS couples phosphorylation reactions with carbohydrate specificity. We have determined the crystal structure of the Escherichia coli mannitol-specific IIA domain, IIAmtl (M(r) 16.3 kDa), by multiple anomalous dispersion analysis of a selenomethionine variant of IIAmtl. The structure was refined at 1.8 A resolution to an R factor of 19.0% (Rfree 24.2%). The enzyme consists of a single five-stranded mixed beta sheet, flanked by helices on both sides. The phosphorylation site (His65) is located at the end of the third beta...Continue Reading

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Citations

Oct 29, 1998·Protein Science : a Publication of the Protein Society·R L van Montfort, B W Dijkstra
Oct 21, 2009·The Journal of Biological Chemistry·Kemin TanAndrzej Joachimiak
Aug 5, 2000·Applied and Environmental Microbiology·R H Ffrench-ConstantF R Blattner
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Aug 12, 2005·BMC Structural Biology·Jingshan RenRaymond J Owens
Aug 18, 2009·Biochemical and Biophysical Research Communications·Stefan ReineltKlaus Scheffzek
Sep 7, 2007·Biochemical and Biophysical Research Communications·Caifang YuChangwen Jin
Aug 8, 2007·Journal of Molecular Biology·Pier Federico GherardiniMichael J E Sternberg
Jun 19, 2012·Biochimica Et Biophysica Acta·Trevor F Moraes, Reinhart A F Reithmeier
Mar 25, 2014·Biochimica Et Biophysica Acta·Jason G McCoyMing Zhou
Jan 20, 2016·Molecular Microbiology·Sonja A WestermayerJoachim O Rädler
Oct 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Malgorzata MagochGrzegorz Dubin

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