PMID: 3758066Sep 1, 1986Paper

The structure of the O-glycosidic oligosaccharide chains of the major Zajdela hepatoma ascites-cell-membrane glycoprotein

European Journal of Biochemistry
F NatoJ F Vliegenthart

Abstract

Glycoprotein MII2, the major cell surface glycoprotein (molecular mass 110 kDa) of Zajdela hepatoma ascites cells, contains about 25 O-glycosidic oligosaccharide chains per molecule. They were released as oligosaccharide-alditols by alkaline borohydride treatment of MII2, and purified by gel filtration on Bio-Gel P-6 followed by high-voltage paper electrophoresis. Four oligosaccharide-alditol fractions (A-D) were obtained in relative yields of 8:6:3:3. The structure of the components of fractions A-C was determined by 500-MHz 1H-NMR spectroscopy in combination with sugar composition analysis, to be as follows. (A) NeuAc alpha(2----3)Gal beta(1----3)[NeuAc alpha(2----3)Gal beta(1----4)GlcNAc beta(1----6)]GalNAc-ol; (B1) NeuAc alpha(2----3)Gal beta(1----3)[Gal beta(1----4)GlcNAc beta(1----6)]GalNAc-ol; (B2) Gal beta(1----3)[NeuAc alpha(2----3)Gal beta(1----4)GlcNAc beta(1----6)]GalNAc-ol; (C) NeuAc alpha(2----3)Gal beta(1----3)GalNAc-ol. On the basis of sugar composition and characteristics on Bio-Gel P-6 filtration, paper electrophoresis and thin-layer chromatography, the structure of the carbohydrate component of fraction D is proposed to be as follows. (D) NeuAc alpha(2----3)Gal beta(1----3)[NeuAc alpha(2----6)]GalNAc-ol

References

Sep 4, 1979·Biochemistry·E P Lennette, B V Plapp
Dec 11, 1979·Biochemistry·E V Chandrasekaran, E A Davidson
Nov 1, 1974·European Journal of Biochemistry·C G Lombart, R J Winzler
May 2, 1984·European Journal of Biochemistry·S A KorrelJ F Vliegenthart
Jan 1, 1984·Biochimica Et Biophysica Acta·L A Smets, W P Van Beek
Feb 27, 1980·Biochemical and Biophysical Research Communications·E F HounsellS Hakomori
Jul 1, 1980·European Journal of Biochemistry·S SoulierL Szabó
Sep 30, 1961·Biochimica Et Biophysica Acta·D F WALLACH, E H EYLAR

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Citations

Aug 24, 2010·Omics : a Journal of Integrative Biology·Albert LeeNicolle H Packer

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