PMID: 8582321Jan 1, 1995Paper

The subdomain structure of human serum albumin in solution under different pH conditions studied by small angle X-ray scattering

European Biophysics Journal : EBJ
J R Olivieri, A F Craievich

Abstract

Small-angle X-ray scattering (SAXS) was used to study structural characteristics of human serum albumin (HSA) in solution under different pH conditions. Guinier analysis of SAXS results yielded values of the molecular radius of gyration ranging from 26.7 A to 34.5 A for pH varying from 2.5 to 7.0. This suggests the existence of significant differences in the overall shape of the molecule at different pH. Molecular models based on subdomains with different spatial configurations were proposed. The distance distribution functions associated with these models were calculated and compared with those determined from the experimental SAXS intensity functions. The conclusion of this SAXS study is that the arrangement of molecular subdomains is clearly pH dependent; the molecule adopting more or less compact configuration for different pH conditions. The conclusions of this systematic study on the modification in molecular shape of HSA as a response to pH changes is consistent with those of previous investigations performed for particular pH conditions.

Citations

Sep 27, 2000·Biochimica Et Biophysica Acta·J R Gillespie, V N Uversky
Dec 25, 2015·The Journal of Physical Chemistry Letters·Nirbhik AcharyaSantosh Kumar Jha
Nov 27, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Richard A CampbellJohn W White
Sep 19, 2003·Biochemical and Biophysical Research Communications·Walter Filgueira de AzevedoDiógenes Santiago Santos
Mar 30, 2010·Journal of Structural Biology·Kim Langmach HeinPoul Nissen
Apr 6, 2013·Biochimica Et Biophysica Acta·Ulrich Kragh-HansenTheodore Peters
Sep 30, 2004·Journal of Dairy Science·H M FarrellH E Swaisgood
Jul 31, 2020·Expert Opinion on Drug Metabolism & Toxicology·Diego Pilati, Kenneth A Howard
May 2, 2018·Clinical Hemorheology and Microcirculation·Christoph DibiasiUrsula Windberger
May 3, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Benjamin J CowsillJian R Lu
Dec 10, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Guangcui YuanSushil K Satija

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