The Subgingival Microbiome of Periodontal Pockets With Different Probing Depths in Chronic and Aggressive Periodontitis: A Pilot Study

Frontiers in Cellular and Infection Microbiology
Meng ShiRuifang Lu

Abstract

Periodontitis is a kind of infectious disease initiated by colonization of subgingival periodontal pathogens, which cause destruction of tooth-supporting tissues, and is a predominant threat to oral health as the most common cause of loss of teeth. The aim of this pilot study was to characterize the subgingival bacterial biodiversity of periodontal pockets with different probing depths in patients with different forms of periodontitis. Twenty-one subgingival plaque samples were collected from three patients with chronic periodontitis (ChP), three patients with aggressive periodontitis (AgP) and three periodontally healthy subjects (PH). Each patient with periodontitis was sampled at three sites, at different probing depths (PDs, one each at 4 mm, 5-6 mm, and ≥ 7 mm). Using 16S rRNA gene high-throughput sequencing and bioinformatic analysis, we found that subgingival communities in health and periodontitis samples largely differed. Meanwhile, Acholeplasma, Fretibacterium, Porphyromonas, Peptococcus, Treponema_2, Defluviitaleaceae_UCG_011, Filifactor, and Mycoplasma increased with the deepening of the pockets in ChP, whilst only Corynebacterium was negatively associated with PD. In AgP, Corynebacterium and Klebsiella were positiv...Continue Reading

References

Jul 1, 1992·Journal of Clinical Periodontology·A D HaffajeeJ M Goodson
Nov 1, 1991·Journal of Clinical Periodontology·S S SocranskyS Dibart
Sep 1, 1984·Journal of Periodontology·G R MettrauxH Graf
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Jun 23, 2000·Annals of Periodontology·G C Armitage
May 15, 2001·Journal of Clinical Periodontology·A D Haffajee, S S Socransky
Aug 1, 1954·Journal of Dental Research·B K FORSCHERW C HESS
Aug 6, 2005·Journal of Clinical Microbiology·Purnima S KumarEugene J Leys
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
Aug 26, 2006·Periodontology 2000·Anne D HaffajeeSigmund S Socransky
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Mar 26, 2010·The ISME Journal·Elisabeth M BikDavid A Relman
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Apr 21, 2010·Periodontology 2000·Gary C Armitage, Mary P Cullinan
Sep 10, 2011·Bioinformatics·Tanja Magoč, Steven L Salzberg
Mar 2, 2012·Archives of Oral Biology·Débora HellerAna Paula Vieira Colombo
May 8, 2014·Environmental Microbiology·Xin XuXuedong Zhou
Jun 28, 2014·Journal of Microbiological Methods·Osamu TsuzukibashiMasatomo Hirasawa
Apr 24, 2015·Journal of Dental Research·O-J ParkS H Han
Sep 29, 2015·Journal of Clinical Periodontology·Yi LiHuanxin Meng
Sep 6, 2017·Journal of Clinical Periodontology·Paula Juliana Pérez-ChaparroMagda Feres
Feb 21, 2018·Human Reproduction·Ming YuanGuoyun Wang

❮ Previous
Next ❯

Citations

Jul 23, 2019·Journal of Dental Research·R J GencoJ Wactawski-Wende
Aug 4, 2019·Journal of Clinical Periodontology·Hongye LuHuanxin Meng
Apr 17, 2020·Journal of Dental Research·T E MatshaG M Davison
May 24, 2020·Journal of Clinical Medicine·Hee Sam NaJin Chung
Mar 12, 2020·NPJ Biofilms and Microbiomes·Li GaoYvonne L Kapila
Mar 28, 2019·Frontiers in Cellular and Infection Microbiology·Yiping WeiXiaolei Wu
Aug 5, 2020·Journal of Clinical Medicine·Lourdes NóvoaPedro Diz
Jul 10, 2019·Frontiers in Cellular and Infection Microbiology·Anna LundmarkTülay Yucel-Lindberg
Mar 28, 2020·International Journal of Environmental Research and Public Health·Silvia TimkováAndrea Madarasová Gecková
Nov 24, 2020·Periodontology 2000·Flavia TelesJulie T Marchesan
Nov 24, 2020·Periodontology 2000·Magda FeresFlavia Teles
Jan 8, 2021·Journal of Periodontal Research·Ethan NgChaminda J Seneviratne
Jun 7, 2020·Medical Hypotheses·Zahi BadranAssem Soueidan
Feb 9, 2021·Experimental Gerontology·Irene YangJames Lah
Oct 29, 2020·Clinical Oral Investigations·Torsten SterzenbachMatthias Hannig
Dec 22, 2020·Frontiers in Pharmacology·Larissa SteigmannWilliam V Giannobile
Mar 19, 2021·Journal of Dental Research·T BeiklerT F Flemmig
Jun 18, 2021·Frontiers in Microbiology·Jessica ChopykLaura E Crotty Alexander
Nov 1, 2020·Archives of Oral Biology·Tamires Szeremeske MirandaPoliana Mendes Duarte

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRP125475

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

QIIME
R
SPSS
QIIME Quantitative Insights Into Microbial Ecology
house Perl scripts
GraphPad PRISM®
FLASH

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.