The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs

Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi
Dan XuYe-Qing Sun

Abstract

Microgravity is known to cause endothelium dysfunction in astronauts returning from spaceflight. We aimed to reveal the regulatory mechanism in alterations of human endothelial cells after simulated microgravity (SMG). We utilized the rotary cell culture system (RCCS-1) to explore the subsequent effects of SMG on human umbilical vein endothelial cells (HUVECs). SMG-treated HUVECs appeared obvious growth inhibition after return to normal gravity, which might be attributed to a set of responses including alteration of cytoskeleton, decreased cell adhesion capacity and increased apoptosis. Expression levels of mTOR and its downstream Apaf-1 were increased during subsequent culturing after SMG. miR-22 was up-regulated and its target genes SRF and LAMC1 were down-regulated at mRNA levels. LAMC1 siRNAs reduced cell adhesion rate and inhibited stress fiber formation while SRF siRNAs caused apoptosis. SMG has the subsequent biological effects on HUVECs, resulting in growth inhibition through mTOR signaling and miR-22-mediated mechanism.

Citations

Apr 30, 2020·International Journal of Molecular Sciences·Xiao LinAirong Qian
Dec 16, 2020·International Journal of Molecular Sciences·Binod PrasadMarcus Krüger
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna ChoromańskaJolanta Saczko
Jul 6, 2021·Frontiers in Bioengineering and Biotechnology·Stefan RiwaldtDaniela Grimm

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Methods Mentioned

BETA
flow cytometry
PCR
transfection
X-ray
GTPases

Software Mentioned

ImageJ
miRanda
Targetscan
Excel
PicTar
Adobe Photoshop

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