The subunit structure of thymus leukemia antigens.

Biochemistry
H AnundiP A Peterson

Abstract

EDTA-containing buffer solubilizes thymus leukemia antigens (TLa) from crude thymocyte membrane fractions. The TL antigens consist mainly of molecules of a size similar to immunoglobulin G when gel chromatography analyses were performed under physiological conditions. A single component of TLa was apparent on sucrose density gradient ultracentrifugation of solubilized thymocyte membrane macromolecules as monitored by indirect immunoprecipitation. The sedimentation constant for the TL antigens (5.8 S) was considerably less than that for immunoglobulin G. The gel chromatography and ultracentrifugation data suggest an apparent molecular weight for TLa of about 120000. TLa isolated by indirect immunoprecipitation is composed of two types of polypeptide chains. The smaller subunit was identified as beta2-microglobulin. The larger polypeptide chain carried the alloantigenic determinants and displayed a molecular weight of about 50000 after reduction and alkylation. TLa subjected to molecular weight determination under denaturing conditions was composed of two components. The smaller component was beta2-microglobulin which evidently is linked to the larger polypeptide chain by noncovalent interactions only. The larger component had a ...Continue Reading

References

Feb 27, 1975·Nature·L OstbergP A Peterson
Jan 1, 1972·Annual Review of Genetics·J A Gally, G M Edelman
May 31, 1974·Nature·L UstbergP A Peterson
Jun 28, 1974·Nature·L RaskP A Peterson
Feb 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation·P E Evrin, L Wibell

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Citations

Jan 1, 1983·Immunogenetics·K YokoyamaS G Nathenson
Jan 1, 1981·Journal of Immunological Methods·R CurryR P Messner
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C TerhorstJ L Strominger
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S KvistP A Peterson
Oct 1, 1979·The Journal of Experimental Medicine·E Rothenberg, E A Boyse
Apr 1, 1978·Scandinavian Journal of Immunology·S KvistP A Peterson
May 17, 1976·Biochemical and Biophysical Research Communications·E AppellaD Pressman
Jan 1, 1979·CRC Critical Reviews in Clinical Laboratory Sciences·M D PoulikJ Shuster

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