The successful induction of T-cell and antibody responses by a recombinant measles virus-vectored tetravalent dengue vaccine provides partial protection against dengue-2 infection

Human Vaccines & Immunotherapeutics
Hui-Mei HuChien-Hsiung Pan

Abstract

Dengue has a major impact on global public health, and the use of dengue vaccine is very limited. In this study, we evaluated the immunogenicity and protective efficacy of a dengue vaccine made from a recombinant measles virus (MV) that expresses envelope protein domain III (ED3) of dengue-1 to 4. Following immunization with the MV-vectored dengue vaccine, mice developed specific interferon-gamma and antibody responses against dengue virus and MV. Neutralizing antibodies against MV and dengue viruses were also induced, and protective levels of FRNT50 ≥ 10 to 4 serotypes of dengue viruses were detected in the MV-vectored dengue vaccine-immunized mice. In addition, specific interferon-gamma and antibody responses to dengue viruses were still induced by the MV-vectored dengue vaccine in mice that were pre-infected with MV. This finding suggests that the pre-existing immunity to MV did not block the initiation of immune responses. By contrast, mice that were pre-infected with dengue-3 exhibited no effect in terms of their antibody responses to MV and dengue viruses, but a dominant dengue-3-specific T-cell response was observed. After injection with dengue-2, a detectable but significantly lower viremia and a higher titer of anti-de...Continue Reading

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Citations

Nov 10, 2016·Vaccines·Kenneth Lundstrom
Jul 16, 2017·Virus Genes·Michael D Mühlebach
Sep 8, 2018·Frontiers in Immunology·Nagesh K Tripathi, Ambuj Shrivastava
Feb 27, 2020·Viruses·Elena BuschChristine E Engeland
Jul 24, 2020·International Journal of Molecular Sciences·Kenneth Lundstrom

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Datasets Mentioned

BETA
AF266287

Methods Mentioned

BETA
flow cytometry
transgenic
ELISA
PCR
transfection
FCS
electrophoresis

Software Mentioned

Treestar
GraphPad Prism
FlowJo
ImmunoSpot

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