The Sucrose Synthase Gene Family in Chinese Pear (Pyrus bretschneideri Rehd.): Structure, Expression, and Evolution

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Muhammad AbdullahYongping Cai

Abstract

Sucrose synthase (SS) is a key enzyme involved in sucrose metabolism that is critical in plant growth and development, and particularly quality of the fruit. Sucrose synthase gene families have been identified and characterized in plants various plants such as tobacco, grape, rice, and Arabidopsis. However, there is still lack of detailed information about sucrose synthase gene in pear. In the present study, we performed a systematic analysis of the pear (Pyrus bretschneideri Rehd.) genome and reported 30 sucrose synthase genes. Subsequently, gene structure, phylogenetic relationship, chromosomal localization, gene duplications, promoter regions, collinearity, RNA-Seq data and qRT-PCR were conducted on these sucrose synthase genes. The transcript analysis revealed that 10 PbSSs genes (30%) were especially expressed in pear fruit development. Additionally, qRT-PCR analysis verified the RNA-seq data and shown that PbSS30, PbSS24, and PbSS15 have a potential role in the pear fruit development stages. This study provides important insights into the evolution of sucrose synthase gene family in pear and will provide assistance for further investigation of sucrose synthase genes functions in the process of fruit development, fruit qua...Continue Reading

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Citations

Feb 26, 2019·Frontiers in Plant Science·Ofer Stein, David Granot
Aug 8, 2020·Computational Biology and Chemistry·Muhammad Aamir ManzoorYongping Cai
Jan 21, 2021·International Journal of Molecular Sciences·Lavinia MareriGiampiero Cai
Mar 8, 2020·International Journal of Biological Macromolecules·Waqar Afzal MalikWuwei Ye

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Datasets Mentioned

BETA
PRJNA309745

Methods Mentioned

BETA
reverse transcription PCR
RNA-seq
PCR

Software Mentioned

ClustalX
ExPASY
tophat2
HMMER
Perl script
circos
PlantCARE
MCScanX
fastx
Beacon Designer

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