The sul1 gene in Stenotrophomonas maltophilia with high-level resistance to trimethoprim/sulfamethoxazole

Annals of Laboratory Medicine
Hae-Sun ChungYunsop Chong

Abstract

Emerging resistance to trimethoprim/sulfamethoxazole (SXT) poses a serious threat to the treatment of Stenotrophomonas maltophilia infections. We determined the prevalence and molecular characteristics of acquired SXT resistance in recent clinical S. maltophilia isolates obtained from Korea. A total of 252 clinical isolates of S. maltophilia were collected from 10 university hospitals in Korea between 2009 and 2010. Antimicrobial susceptibility was determined by using the CLSI agar dilution method. The sul1, sul2, and sul3 genes, integrons, insertion sequence common region (ISCR) elements, and dfrA genes were detected using PCR. The presence of the sul1 gene and integrons was confirmed through sequence analysis. Among the 32 SXT-resistant isolates, sul1 was detected in 23 isolates (72%), all of which demonstrated high-level resistance (≥64 mg/L) to SXT. The sul1 gene (varying in size and structure) was linked to class 1 integrons in 15 of the 23 isolates (65%) harboring this gene. None of the SXT-susceptible isolates or the SXT-resistant isolates with a minimum inhibitory concentration of 4 and 8 mg/L were positive for sul1. Moreover, the sul2, sul3, and dfrA genes or the ISCR elements were not detected. The sul1 gene may play ...Continue Reading

References

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Citations

Aug 17, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Kenia Barrantes, Rosario Achí
Apr 4, 2018·Antimicrobial Agents and Chemotherapy·María Blanca Sánchez, José Luis Martínez
Aug 5, 2017·Journal of Medical Microbiology·Sandra Abril Herrera-HerediaSamantha Flores-Treviño
Feb 14, 2020·Expert Review of Anti-infective Therapy·Teresa Gil-GilPaula Blanco
Feb 16, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Miaoli WangJing Chen
Jan 20, 2018·Microbiology Spectrum·Yang WangCongming Wu
May 28, 2021·Clinical Microbiology Reviews·Joanna S Brooke
Aug 3, 2019·Environmental Science & Technology·Hong-Tao WangXi-Mei Xue

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Methods Mentioned

BETA
PCR

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