PMID: 7334024Dec 1, 1981Paper

The sulfhydryl groups of the 35,000-dalton C-terminal segment of band 3 are located in a 9000-dalton fragment produced by chymotrypsin treatment of red cell ghosts

Journal of Bioenergetics and Biomembranes
M RamjeesinghA Rothstein

Abstract

Five sulfhydryl groups of band 3, the anion-transport protein of the red blood cell membrane, can be labeled by N-ethylmaleimide (NEM). Two of these are located in a 35,000-dalton, C-terminal segment produced by chymotrypsin treatment of cells. Extensive treatment of unsealed ghosts with chymotrypsin results in the disappearance of the 35,000-dalton segment, but its two NEM-binding sites area preserved in a 9000-dalton peptide. The latter must therefore be a proteolytic product of the larger segment. Labeling of sulfhydryl groups of band 3 by an impermeant analog of NEM occurs in inside-out, but not in right-side-out vesicles derived from red cell ghosts, supporting the conclusion that NEM-reactive sulfhydryl groups, including those in the 35,000- and 9000-dalton segments, are exposed at the cytoplasmic face of the membrane. These findings support the conclusion that the 35,000-dalton segment crosses the bilayer, and suggest that the 9000-dalton segment may be a membrane-crossing portion of the 35,000-dalton segment.

References

Jan 1, 1977·Journal of Supramolecular Structure·G Guidotti
Feb 21, 1978·Biochimica Et Biophysica Acta·S GrinsteinA Rothstein
May 1, 1991·Enzyme and Microbial Technology·E Y ShamiM Zywulko
Jan 1, 1972·Annual Review of Biochemistry·G Guidotti
Jan 1, 1980·Annals of the New York Academy of Sciences·A Rothstein, M Ramjeesingh
Apr 1, 1984·The Journal of Clinical Investigation·W J KnowlesV T Marchesi
Jan 1, 1980·Annals of the New York Academy of Sciences·M J TannerR E Jenkins
Dec 15, 1980·The Journal of Membrane Biology·M RamjeesinghA Rothstein
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·J S MorrowV T Marchesi

❮ Previous
Next ❯

Citations

Apr 29, 1992·Biochimica Et Biophysica Acta·Z H Zhang, A K Solomon
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S E LuxH F Lodish
Jan 1, 1983·Annals of the New York Academy of Sciences·A K SolomonA S Verkman
Sep 18, 1987·Biochimica Et Biophysica Acta·D M LiebermanR A Reithmeier
Jan 25, 1984·Biochimica Et Biophysica Acta·M RamjeesinghA Rothstein
Jul 10, 1989·Biochimica Et Biophysica Acta·P K WernerR A Reithmeier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.