The sulfhydryl reagent thimerosal elicits human platelet aggregation by mobilization of intracellular calcium and secondary prostaglandin endoperoxide formation

Biochemical and Biophysical Research Communications
M HeckerV Ullrich

Abstract

The effect of the sulfhydryl (SH) group inhibitor ethylmercurithiosalicylate (thimerosal) on the function of human platelets was investigated. In contrast to known SH reagents such as p-chloromercuribenzoate or N-ethylmaleimide, thimerosal elicited both aggregation and [3H]serotonin release of washed human platelets at low micromolar concentrations (greater than or equal to 2 microM). Only a significant higher dose (greater than or equal to 15 microM) was effective when platelets were pretreated with the cyclooxygenase inhibitor aspirin, indicating an amplification of the proaggregatory effect of thimerosal by secondary prostaglandin (PG) endoperoxide and/or thromboxane (TX) formation. Consistent with this notion, thimerosal induced endogenous platelet arachidonic acid (20:4) metabolism which could be attributed to enhanced 20:4 liberation, presumably by activation of phospholipase A2. The latter effect was mediated by mobilization of intracellular calcium (Ca2+), and was not affected by removal of extracellular Ca2+. In the presence of aspirin, the thimerosal-induced Ca2+ elevation was completely reversed by dithiothreitol (DTT) which implicates SH groups in intracellular Ca2+ transport. In contrast to previous observations wi...Continue Reading

References

Feb 15, 1977·Biochemical Pharmacology·D E MacIntyreJ L Gordon
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G de NucciJ R Vane
Aug 1, 1987·Biochemical Pharmacology·J ChangH McGregor
Jan 1, 1986·Annual Review of Cell Biology·U Kikkawa, Y Nishizuka
Apr 15, 1982·The Biochemical Journal·R F Irvine

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Citations

Feb 1, 1997·Molecular Reproduction and Development·H WuR A Fissore
Jan 1, 1994·Pflügers Archiv : European journal of physiology·A DiarraR Sauvé
Oct 19, 1992·Biochimica Et Biophysica Acta·A S GukovskayaV V Bezuglov
Jan 1, 1989·Progress in Lipid Research·H AritaK Hanasaki
Oct 29, 1993·Biochimica Et Biophysica Acta·Y Tsunoda
Dec 1, 1990·Baillière's Clinical Endocrinology and Metabolism·J A Gasser, D J Betteridge
Feb 1, 1993·Zygote : the Biology of Gametes and Early Embryos·A McDougallM Whitaker
Jun 15, 1991·Biochemical Pharmacology·B BrüneV Ullrich
Jun 1, 1992·Thrombosis Research·P V VrzheshchS D Varfolomeyev
Dec 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K R Bruckdorfer
Sep 1, 1992·Thrombosis Research·P V VrzheshchS D Varfolomeyev
Jan 27, 1992·FEBS Letters·M S IslamP O Berggren
Jul 17, 1998·Experimental Cell Research·S J LeeS S Shen

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