The supercoiling sensitivity of a bacterial tRNA promoter parallels its responsiveness to stringent control

The EMBO Journal
N Figueroa-BossiL Bossi

Abstract

In Salmonella typhimurium, expression of the hisR locus, a tRNA operon, decreases upon inhibiting DNA gyrase. Here, the hisR promoter dependence on negative DNA supercoiling was examined in vivo and in vitro. Mutant analysis showed the sequence determinants of this dependence to lie in the region between the -10 box and the transcription start site. As with most promoters subject to stringent control, this portion of the hisR promoter is C-G-rich. Replacing a C/G bp with T/A at position -7 partially relieves the supercoiling response while changing the sequence between -5 and + 1 (-CCCCCG-) for -GTTAA- abolishes the response in vitro and in vivo. The relief of the supercoiling dependence closely correlates with increased promoter susceptibility to melting in vivo and a lesser requirement for initiating nucleotides in the formation of stable initiation complexes in vitro. Studies in isoleucine-starved cells showed that such sequence changes mitigate and abolish the hisR promoter response to stringent control, respectively. The data presented suggest that the hisR promoter's sensitivity to stringent regulation arises from the same physical property that confers supercoiling sensitivity, i.e. resistance to melting. We propose that...Continue Reading

References

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Citations

Jul 31, 2003·Journal of Molecular Biology·Helge AunerAndrew Travers
Mar 30, 2001·Biochimie·A TraversG Muskhelishvili
May 14, 1999·Current Opinion in Microbiology·T Ferenci
Feb 3, 2005·Nature Reviews. Microbiology·Andrew Travers, Georgi Muskhelishvili
Mar 19, 2002·EMBO Reports·Mark RochmanGeorgi Muskhelishvili
Feb 16, 2002·The EMBO Journal·William NasserGeorgi Muskhelishvili
Dec 4, 2012·Journal of Bacteriology·Vikram Vijayan, Erin K O'Shea
Oct 10, 2001·Journal of Bacteriology·M M Barker, R L Gourse
May 6, 2008·Annual Review of Microbiology·Katarzyna Potrykus, Michael Cashel
Apr 28, 2005·Molecular Microbiology·Richard A SteinN Patrick Higgins
May 25, 2010·FEMS Microbiology Reviews·Umender K Sharma, Dipankar Chatterji
Apr 28, 2004·Cell·Irina ArtsimovitchDmitry G Vassylyev
Dec 5, 2016·Nucleic Acids Research·Robert ŁyżeńAgnieszka Szalewska-Pałasz
Aug 1, 2009·EcoSal Plus·Malcolm E Winkler, Smirla Ramos-Montañez

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