PMID: 3756002Jul 1, 1986Paper

The supply of both CDP-choline and diacylglycerol can regulate the rate of phosphatidylcholine synthesis in HeLa cells

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
P LimD E Vance

Abstract

The incorporation of [methyl-14C]CDP-choline into phosphatidylcholine was measured in HeLa cells permeabilized with 0.125 mg digitonin/mL. The rate of phosphatidylcholine formation was influenced by the concentration of CDP-choline in the medium. The CDP-choline:1,2-diacylglycerol cholinephosphotransferase in permeabilized cells showed a Km of 88 microM for CDP-choline. A similar Km value of 104 microM was found for cholinephosphotransferase in microsomes isolated from HeLa cells when assayed in the presence of 2.4 mM dioleoylglycerol. In the absence of added diacylglycerol, the Km for CDP-choline for the microsomal cholinephosphotransferase was only 38 microM. The incorporation of [methyl-14C]CDP-choline into phosphatidylcholine was stimulated by the supply of diacylglycerol in both HeLa cells and isolated microsomes. A 2.4 mM dioleoylglycerol suspension increased cholinephosphotransferase activity fourfold in microsomes. The digitonin-treated cells were impermeable to the dioleoylglycerol suspension. Incubation of permeabilized cells with 150 microM acyl-CoA and 0.8 mM glycero-3-phosphate tripled cellular diacylglycerol levels, causing a doubling in the rate of phosphatidylcholine synthesis. A similar incubation of microsomes...Continue Reading

Citations

May 8, 2008·Current Opinion in Lipidology·Dennis E Vance
Apr 30, 2009·Annual Review of Nutrition·Richard J WurtmanIsmail H Ulus
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·W Araki, R J Wurtman
Mar 9, 2011·Progress in Lipid Research·Martin HermanssonPentti Somerharju
Jul 22, 2008·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Mehmet CansevIsmail H Ulus
Jan 29, 2013·Critical Reviews in Biochemistry and Molecular Biology·Neale D Ridgway
Jan 11, 2007·The Journal of Biological Chemistry·Paolo FagoneSuzanne Jackowski
Jul 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E Fleischer-LambropoulosA Vernadakis
Jun 9, 1988·Biochimica Et Biophysica Acta·M Post, L M van Golde

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