The suppressive effect of soluble Klotho on fibroblastic growth factor 23 synthesis in UMR-106 osteoblast-like cells

Cell Biology International
Lulu MaChangying Xing

Abstract

Fibroblastic growth factor 23 (FGF23) is a hormone secreted primarily by bone. FGF23 is elevated in the serum of chronic kidney disease (CKD) patients, but the exact mechanism is not well known. Klotho is identified as an aging suppressor, which is mainly expressed in the kidney, and the level of soluble Klotho is negatively associated with FGF23 in CKD. The aim of this study was to investigate the effect and possible mechanism of Klotho on FGF23 synthesis in osteoblast-like UMR-106 cells. UMR-106 cells were divided into five groups: (i) control group; (ii) β-glycerophosphate (β-GP) group; (iii) β-GP + Klotho group; (iv) β-GP+ lithium chloride (LiCl, a Wnt/β-catenin pathway agonist) group; and (v) β-GP + Klotho + LiCl group. Subsequently, UMR-106 cells were cultured for 72 h, and the expression of FGF23, P-glycogen synthase kinase-3β (P-GSK-3β), and glycogen synthase kinase-3β(GSK-3β) were measured with Western blot analysis. The mRNA levels of FGF23 and the Wnt/β-catenin pathway target gene c-myc were determined with RT-qPCR. The results showed that β-GP induced increased expression of FGF23 mRNA and protein. Compared with the β-GP group, expression of FGF23 mRNA and protein expression were downregulated in the β-GP + Klotho g...Continue Reading

References

Jan 27, 2006·The Journal of Biological Chemistry·Hiroshi KurosuMakoto Kuro-o
Nov 7, 2006·Nature·Itaru UrakawaTakeyoshi Yamashita
Aug 11, 2007·Science·Hongjun LiuToren Finkel
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
May 29, 2010·Journal of the American Society of Nephrology : JASN·Myles Wolf
Mar 8, 2012·Advances in Experimental Medicine and Biology·Ming Chang HuOrson W Moe
Sep 18, 2012·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Lynda F Bonewald, Michael J Wacker
Oct 23, 2012·Biochemical and Biophysical Research Communications·Ningyuan SunQuan Yuan
Apr 6, 2013·Journal of the American Society of Nephrology : JASN·Lili ZhouYouhua Liu
Feb 6, 2014·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Hyung Jung OhSeung Hyeok Han
Sep 17, 2014·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·L-N ZhangN-N Wang
May 16, 2015·Journal of the American Society of Nephrology : JASN·Ming Chang HuOrson W Moe
Jan 17, 2016·Biochemical and Biophysical Research Communications·Bingbing ZhangFlorian Lang
Feb 12, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jovana KaludjerovicBeate Lanske

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Related Papers

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Ivana PavikAndreas L Serra
Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Mandy WanRukshana Shroff
© 2022 Meta ULC. All rights reserved