The suppressor of cytokine signaling (SOCS)-7 interacts with the actin cytoskeleton through vinexin

Experimental Cell Research
Nele MartensElisabeth L Hooghe-Peters

Abstract

To understand the function of the suppressor of cytokine signaling (SOCS)-7, we have looked for proteins interacting with SOCS-7 in a stringent yeast two-hybrid screen of a human leukocyte cDNA-library. We identified the cytoskeletal molecule vinexin as a partner interacting with SOCS-7. Tests with deletion mutants of SOCS-7 demonstrated that a central region of the molecule containing several proline-rich regions, N-terminal to the SH2 domain, was responsible for the binding to vinexin. It is thus likely that one of the SH3 domains of vinexin interacts with a poly-proline region of SOCS-7. The interaction with vinexin was confirmed biochemically as vinexin-alpha was co-precipitated with SOCS-7. Confocal laser-scanning microscopy in HEK293T, MCF-7, and 3T3-L1 cells showed that part of the transfected SOCS-7-green fluorescent protein (GFP) molecules merged with vinexin and with actin. Taken together, our data indicate that SOCS-7 interacts with vinexin and the actin cytoskeleton.

References

Nov 5, 1997·Biochemical and Biophysical Research Communications·K MatuokaT Takenawa
Oct 6, 1998·Annual Review of Biochemistry·I W Mattaj, L Englmeier
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J G ZhangM Baca
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·A FloresM Werner
Nov 10, 2000·Biochemical and Biophysical Research Communications·B R DeyP Nissley
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·A KimuraA R Saltiel
Dec 26, 2001·The American Journal of Pathology·C A FrenchJ A Fletcher
Dec 19, 2001·The Journal of Clinical Investigation·C J Auernhammer, S Melmed
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Silvia M GoicoecheaChuanyue Wu
Jun 8, 2002·Molecular and Cellular Biology·Danielle L KrebsWarren S Alexander
Jun 22, 2002·Trends in Biochemical Sciences·Benjamin T KileDouglas J Hilton
Sep 14, 2002·The Journal of Biological Chemistry·Liangyou RuiMorris F White
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoh-Ichi SekiMasato Kubo
May 20, 2003·Nature Immunology·Ben A CrokerWarren S Alexander
Jul 29, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Filip BraetDavid G Le Couteur
Aug 7, 2003·Current Opinion in Pharmacology·Kyoko Inagaki-OharaAkihiko Yoshimura
Sep 23, 2003·Science·Peter W KimMichael J Eck

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Citations

Apr 24, 2014·Molecular Biology International·Walid SasiKefah Mokbel
Oct 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Danielle L KrebsWarren S Alexander
Apr 8, 2014·Cellular and Molecular Life Sciences : CMLS·Julhash U KaziLars Rönnstrand
Aug 31, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Nuzhat N KabirJulhash U Kazi
Aug 30, 2005·The Journal of Clinical Investigation·Alexander S BanksPaul B Rothman
Feb 4, 2009·Cell Communication and Signaling : CCS·Marcus LettauOttmar Janssen
Jan 5, 2010·Developmental and Comparative Immunology·Timothée BruelFrançois Meurens
Apr 10, 2007·Experimental Cell Research·Masaru MitsushimaNoriyuki Kioka
Jan 30, 2007·The Journal of Allergy and Clinical Immunology·Judit Knisz, Paul B Rothman
Sep 17, 2009·Advances in Immunology·Suzanne L Cassel, Paul B Rothman
Mar 18, 2015·Molecular and Cellular Biology·Jonathan A CooperShawn S C Li
Sep 17, 2005·Journal of Cellular Biochemistry·Elena Faraci-OrfWolfgang F Vogel
Apr 15, 2009·Physiology·Michael S HuhDavid J Picketts

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