The SURF-6 protein is a component of the nucleolar matrix and has a high binding capacity for nucleic acids in vitro

European Journal of Cell Biology
C MagoulasM Fried

Abstract

The recently identified novel protein SURF-6 is shown to be a component of the nucleolar matrix. Immunofluorescence analysis demonstrated that SURF-6 was localized in residual nucleoli of in situ nuclear matrix preparations of mouse fibroblast cells (NIH 3T3), which were depleted of soluble and chromatin related proteins. Immunoblot analysis of biochemical nucleolar subfractions confirmed that SURF-6 was present in the nucleolar matrix fraction, and was absent from the fractions of soluble proteins released by DNase or RNase. The capacity of SURF-6 to bind nucleic acids was investigated in vitro. Both endogenous SURF-6 from nuclear extracts and recombinant SURF-6 exhibited a strong binding capacity for nucleic acids. It was shown that SURF-6 bound to both DNA and RNA, however, it showed stronger binding to RNA. The presence and nuclear distribution of SURF-6 during the cell cycle was explored by immunofluorescence analysis. It was shown that SURF-6 was always found in the nucleolus regardless of the phase of the cell cycle suggesting that it is a structural protein constitutively present in nucleolar substructures. The colocalization of SURF-6 with the major nucleolar proteins B23 and fibrillarin, which are known to be involved...Continue Reading

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Citations

Oct 1, 2011·Functional & Integrative Genomics·Mikhail A Kutuzov, Alexandra V Andreeva
Nov 13, 2009·Bulletin of Experimental Biology and Medicine·A A MoralevaO V Zatsepina
Jan 20, 2004·BMC Neuroscience·Sanja Selak, Marvin J Fritzler
May 6, 2014·Doklady. Biochemistry and Biophysics·M Yu KordyukovaO V Zatsepina
Sep 2, 2005·Chromosoma·Aaron A Van HooserRebecca Heald
Jan 5, 2005·Biochemical and Biophysical Research Communications·Mikhail PolzikovCharalambos Magoulas
Jul 21, 2006·Biology of Reproduction·Ludmila G RomanovaRichard M Schultz
Feb 28, 2018·Nature Communications·Diana M MitreaRichard W Kriwacki
Dec 21, 2005·Bioorganicheskaia khimiia·V V GurchenkovO V Zatsepina
Nov 7, 2006·Molecular Biology Reports·Mikhail PolzikovOlga Zatsepina

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