The surface adhesion molecule CXCR4 stimulates mesenchymal stem cell migration to stromal cell-derived factor-1 in vitro but does not decrease apoptosis under serum deprivation

Cardiovascular Revascularization Medicine : Including Molecular Interventions
Shyam BhaktaOmer Koc

Abstract

Bone marrow mesenchymal stem cells (MSCs) can be used for myocardial repair following myocardial infarction. Increased expression of stromal cell-derived factor-1 (SDF-1) by an ischemic myocardium attracts CXCR4+ stem cells toward it. CXCR4, the receptor for SDF-1, is important in the migration, homing, and survival of hematopoietic stem cells. Although low levels of CXCR4 expression were found in minor subpopulations of cultured MSCs, most MSCs do not express CXCR4. To optimize the migration and survival of human MSCs, we expressed the CXCR4 gene in these cells using retroviral transduction. We isolated and cultured MSCs from healthy volunteers and transduced them with a retroviral vector containing either CXCR4 and green fluorescent protein (GFP; CXCR4/GFP vector) or GFP alone (control vector). Flow cytometry confirmed successful transduction and GFP and CXCR4 expression. We used a transwell migration system to study MSC migration to SDF-1. We used Annexin V and propidium iodide stains to assess cell survival before and after the survival challenge. Flow cytometry showed that, on average, 83.4+/-17.7% of transduced MSCs expressed CXCR4. Compared with control MSCs, MSCs transduced with CXCR4 showed significantly more migration...Continue Reading

References

Sep 4, 2003·Circulation·James S ForresterRaj R Makkar

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Citations

Apr 25, 2007·Cell and Tissue Research·Andrea T BadilloKenneth W Liechty
Oct 13, 2010·Journal of Molecular Neuroscience : MN·Naghmeh Ahmadian KiaHamid Reza Bidkhori
Feb 7, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Zhaokang ChengDeling Kong
Feb 13, 2008·The Journal of Experimental Medicine·Benedetta MazzinghiPaola Romagnani
Apr 13, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Jul 8, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Jun 6, 2009·World Journal of Gastroenterology : WJG·Shi-Zhu JinBing-Rong Liu
Feb 19, 2009·Stem Cell Reviews and Reports·Danijela MenicaninStan Gronthos
Jan 12, 2011·Expert Opinion on Biological Therapy·Ting Ting Lau, Dong-An Wang
Aug 30, 2011·The Veterinary Clinics of North America. Equine Practice·Matthew C Stewart
Apr 24, 2010·Neuroscience Research·Shiyong LiYe Wang
Sep 29, 2009·Current Opinion in Biotechnology·Joseph WagnerArnold I Caplan
Jul 19, 2015·Immunology Letters·Anne S CornelissenCarlijn Voermans
Jan 19, 2008·Journal of Molecular and Cellular Cardiology·Dongsheng ZhangYigang Wang
Aug 21, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jason ChamberlainGraça Almeida-Porada
Aug 1, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J LiZ Song
Dec 15, 2010·Journal of Cellular and Molecular Medicine·Hongwei ShaoHong Yu
Nov 30, 2012·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Zhiyong DuYingbin Liu
Nov 10, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·L ZhangA Wang
Oct 30, 2009·British Journal of Haematology·Marijke W MaijenburgCarlijn Voermans
Jul 29, 2015·Clinical and Experimental Pharmacology & Physiology·Gila Pirzad JahromiJason Charish
Mar 28, 2008·Cytotherapy·S Bhakta, M J Laughlin
Jan 26, 2016·The Ocular Surface·Vivien J Coulson-ThomasWinston W-Y Kao

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